Fathi Mokhtar, Zarrinkavyani Kianoosh, Biranvand Zahra, Abd Morteza Aghil Al
Department of Animal Science, Payame Noor University, Tehran, Iran.
Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran.
Poult Sci. 2025 May 8;104(10):105265. doi: 10.1016/j.psj.2025.105265.
This experiment was conducted to investigate the effects of dietary phloretin on broiler chickens under pulmonary arterial hypertension (PAH) conditions. A total of 500 one-day-old male broiler chickens were randomly allocated into five treatments with five replicate pens containing 20 birds per pen. The negative control group was reared at normal temperature and fed with basal diet while the PAH-treatments (positive control, F-200, F-400, and F-600) were exposed to low temperatures and received drinking water supplemented with excess salt to induce PAH and fed with basal diet containing 0, 200, 400, and 600 mg of phloretin per kilogram, respectively. Blood samples were collected from broilers at 42 days of age. Results showed that PAH decreased body weight gain (BWG), antioxidant capacity, immunoglobulin G (IgG), hematological indices, and cecal lactic acid bacteria population (CLBP), but increased feed intake (FI), PAH-related mortality, feed conversion ratio (FCR), PAH index, and pro-inflammatory cytokines, alanine transaminase (ALT), aspartate transaminase (AST), triglyceride (TG), and total cholesterol (TC) contents in serum (P<0.001). Among the PAH treatments, supplementary phloretin improved growth performance indices and reduced PAH index, and PAH-related mortality (P < 0.01). The activities of superoxide dismutase, catalase, and glutathione peroxidase, and IgG, and IgM in serum were increased, and malondialdehyde was reduced by phloretin supplementation (P <0.01). The PAH-induced effect on contents of pro-inflammatory cytokines were reduced by dietary phloretin supplementation (P <0.01). Moreover, supplementation of diets with phloretin alleviated the adverse effect of PAH as reflected by a reduction in ALT, AST, TG, and TC (P <0.01). The red blood cell count, hemoglobin, hematocrit, and heterophil levels reduced by phloretin supplementation. Phloretin increased the population of CLBP. In conclusion, phloretin supplementation during PAH may mitigate PAH-associated physiological and biochemical alterations in broiler chickens.
本试验旨在研究日粮根皮素对肺动脉高压（PAH）条件下肉鸡的影响。将500只1日龄雄性肉鸡随机分为5个处理组，每个处理组设5个重复栏，每栏20只鸡。阴性对照组在常温下饲养，饲喂基础日粮，而PAH处理组（阳性对照组、F - 200、F - 400和F - 600）暴露于低温环境，饮用添加过量盐分的水以诱导PAH，并分别饲喂每千克含0、200、400和600毫克根皮素的基础日粮。在肉鸡42日龄时采集血样。结果表明，PAH降低了体重增加（BWG）、抗氧化能力、免疫球蛋白G（IgG）、血液学指标和盲肠乳酸菌数量（CLBP），但增加了采食量（FI）、PAH相关死亡率、饲料转化率（FCR）、PAH指数以及血清中促炎细胞因子、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）甘油三酯（TG）和总胆固醇（TC）的含量（P<0.001）。在PAH处理组中，补充根皮素可改善生长性能指标，降低PAH指数和PAH相关死亡率（P<0.01）。补充根皮素可提高血清中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性以及IgG和IgM的水平，并降低丙二醛含量（P<0.01）。日粮中补充根皮素可降低PAH对促炎细胞因子含量的影响（P<0.01）。此外，日粮中补充根皮素可减轻PAH的不良影响，表现为ALT、AST、TG和TC的降低（P<0.01）。补充根皮素可降低红细胞计数、血红蛋白、血细胞比容和嗜异性粒细胞水平。根皮素增加了CLBP的数量。总之，在PAH期间补充根皮素可能减轻肉鸡PAH相关的生理和生化改变。
