Giacomini Marina Ciccone, Wang Linda, de Souza Isabelle Ferreira, Justo Andreza Peres, Honório Heitor Marques, Rios Daniela
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Department of Surgery, Stomatology, Pathology, and Radiology, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
J Dent. 2025 Nov;162:106026. doi: 10.1016/j.jdent.2025.106026. Epub 2025 Aug 8.
Preventive efforts and early diagnosis of erosive tooth wear (ETW) are essential to minimize damage to dental hard tissues. In addition to controlling etiological factors, the synergistic action of specific agents can help maintain oral health. This study aimed to evaluate the ability of bioactive varnishes to prevent and/or control the progression of ETW on enamel.
A total of 120 bovine enamel blocks (n = 5 per subgroup) were randomly allocated into three initial enamel condition groups: sound (S), extrinsically eroded (EE; subjected to orange juice for 1 min, 3×/day, over 5 days), and intrinsically eroded (IE; exposed to 0.01 M hydrochloric acid for 2 min, 4×/day, over 5 days). Each group was subsequently subdivided according to the treatment received: Duraphat (D) - fluoride varnish; Clinpro (C) - tricalcium phosphate-modified formula; and PRG Barrier Coat (B) - containing S-PRG particles. Surface microhardness (SM) (KHN, 25 g/10 s/5×) and wettability (W) were assessed at three time points: I - after the first erosive challenge, AA - after varnish application, and F - after the second erosive challenge. Data were analyzed using normality tests, three-way repeated measures ANOVA, and Tukey's test (α = 0.05).
Both erosive protocols reduced SM compared to the sound group. After the second challenge, all varnishes preserved SM, indicating protective effects. For wettability, intrinsic eroded group showed the lowest values, while varnish PRG Barrier Coat showed the highest.
Bioactive varnishes act as protective barriers against acidic challenges, and their distinct compositions may contribute to the prevention of enamel erosion progression.
Bioactive varnishes contribute to enamel protection by preserving surface hardness under both intrinsic and extrinsic acid exposure.
侵蚀性牙磨损（ETW）的预防措施和早期诊断对于将牙体硬组织的损害降至最低至关重要。除了控制病因外，特定药剂的协同作用有助于维持口腔健康。本研究旨在评估生物活性清漆预防和/或控制牙釉质上ETW进展的能力。
总共120个牛牙釉质块（每个亚组n = 5）被随机分为三个初始牙釉质状况组：完好（S）、外源性侵蚀（EE；每天3次，每次暴露于橙汁1分钟，持续5天）和内源性侵蚀（IE；每天4次，每次暴露于0.01 M盐酸2分钟，持续5天）。随后，根据接受的治疗将每组再细分：Duraphat（D）——含氟清漆；Clinpro（C）——磷酸三钙改性配方；以及PRG屏障涂层（B）——含S-PRG颗粒。在三个时间点评估表面显微硬度（SM）（努氏硬度，25 g/10 s/5次）和润湿性（W）：I——第一次侵蚀性刺激后，AA——涂抹清漆后，F——第二次侵蚀性刺激后。使用正态性检验、三因素重复测量方差分析和Tukey检验（α = 0.05）对数据进行分析。
与完好组相比，两种侵蚀方案均降低了SM。在第二次刺激后，所有清漆均保持了SM，表明具有保护作用。对于润湿性，内源性侵蚀组的值最低，而PRG屏障涂层清漆的值最高。
生物活性清漆可作为抵御酸性刺激的保护屏障，其不同的成分可能有助于预防牙釉质侵蚀的进展。
生物活性清漆通过在内外源性酸暴露下保持表面硬度，有助于牙釉质保护。
