Dos Santos Azevedo Cassia, Faria Júlio Cézar Tannure, Caldeira Marcos Vinicius Winckler, de Oliveira Godinho Tiago, Dos Santos Heloisa Oliveira, Pereira Wilson Vicente Souza, Gomes Gabriel Soares Lopes, Momolli Dione Richer, de Moura Cristiane Coelho, Atala Laura Ribeiro
Department of Forest and Wood Sciences, Federal University of Espírito Santo, Alegre, Espirito Santo Brazil.
Department of Environmental Sciences, Federal University of São Carlos, Sorocaba, São Paulo 18052-780 Brazil.
3 Biotech. 2025 Sep;15(9):289. doi: 10.1007/s13205-025-04463-7. Epub 2025 Aug 8.
Most woody species undergo morphological, physiological, and biochemical changes during ontogenetic development from juvenile to adult phases, especially in terms of clonal potential, growth vigor, and adventitious rooting capacity. This study focused on generating information to support the vegetative propagation of adult individuals of spp., aiming to optimize the mechanisms involved in the induction of adventitious rooting and its association with indole-3-butyric acid (IBA). The experiment evaluated the rejuvenation/reinvigoration of plant tissues from three species by comparing juvenile and adult materials, epicormic shoots, and tissues rescued from the base of felled trees. In contrast to most studies, which focus primarily on juvenile materials, this is the first to investigate cloning through the rescue of adult genotypes, targeting the multiplication of individuals expressing superior phenotypic traits in the field. The results indicated that IBA application did not significantly influence survival, rooting, or the occurrence of abnormalities in the cuttings. Although adventitious rooting was achieved for all species, the percentages did not exceed 30%, reflecting typical results for species with low propagation potential. Basal cuttings showed enzymatic activities of superoxide dismutase, catalase, and peroxidase similar to those observed in juvenile materials, although total protein and carbohydrate contents were less significant. Based on these findings, the quantification of superoxide dismutase and catalase is recommended as physiological indicators of rejuvenation for , while superoxide dismutase is recommended for and . The biochemical differences observed among the materials suggest a strong relationship with the degree of physiological maturation and the expression of antioxidant mechanisms, providing important information for future research into clonal propagation and genetic improvement of spp. Strategies such as successive propagation cycles of rescued materials may contribute to increased adventitious rooting percentages and, consequently, to the rejuvenation/reinvigoration of tissues.
大多数木本植物在从幼年到成年阶段的个体发育过程中会经历形态、生理和生化变化，尤其是在克隆潜力、生长活力和不定根生根能力方面。本研究旨在生成信息以支持 spp. 成年个体的营养繁殖，旨在优化不定根诱导所涉及的机制及其与吲哚 - 3 - 丁酸（IBA）的关联。该实验通过比较幼年和成年材料、隐芽以及从砍伐树木基部挽救的组织，评估了三种 物种植物组织的复壮/活力恢复情况。与大多数主要关注幼年材料的研究不同，这是首次通过挽救成年基因型来研究克隆，目标是繁殖在田间表现出优良表型性状的个体。结果表明，IBA 的应用对插条的存活、生根或异常发生没有显著影响。尽管所有物种都实现了不定根生根，但生根率不超过 30%，这反映了繁殖潜力低的物种的典型结果。基部插条的超氧化物歧化酶、过氧化氢酶和过氧化物酶的酶活性与幼年材料中观察到的相似，尽管总蛋白和碳水化合物含量较低。基于这些发现，建议将超氧化物歧化酶和过氧化氢酶的定量作为 的复壮生理指标，而对于 和 建议使用超氧化物歧化酶。材料之间观察到的生化差异表明与生理成熟程度和抗氧化机制的表达有很强的关系，为未来 spp. 的克隆繁殖和遗传改良研究提供了重要信息。诸如对挽救材料进行连续繁殖循环等策略可能有助于提高不定根生根率，从而促进组织的复壮/活力恢复。
