7,12-二甲基苯并[a]蒽光产物的特性及其对培养的鸡胚细胞的影响。

Characterization of a photoproduct of 7,12-dimethylbenz[alpha]anthracene and its effects on chick-embryo cells in culture.

作者信息

Warshawsky D, Kerns E, Bissell M J, Calvin M

出版信息

Biochem J. 1977 Jun 15;164(3):481-6. doi: 10.1042/bj1640481.

DOI:10.1042/bj1640481

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164822/

Abstract

A common impurity of 7,12-dimethylbenz[alpha]anthracene was more effective than 7,12-dimethylbenz[alpha]anthracene in inducing morphological alterations, and in causing an increase in glucose uptake, DNA synthesis and cell number in chick-embryo fibroblasts. Gradual morphological transformation follows the increase in DNA synthesis after 2 days when either primary or secondary cultures are treated with 3 microgram of the compound/ml. The compound, isolated from 7,12-dimethylbenz[alpha]anthracene by alumina column chromatography, was characterized by t.l.c., mass spectroscopy, carbon-hydrogen analysis, u.v. and nuclear-magnetic-resonance spectroscopy and thermal decomposition. It was the photo-oxidation product of 7,12-dimethylbenz[alpha]anthracene, 7,12-epidioxy-7,12-dimethylbenz[alpha]anthracene. It is suggested that some of the biological effects observed after treatment of cultures with 7,12-dimethylbenz[alpha]anthracene may be due in part to the presence of the photo-oxidation product.

摘要

7,12-二甲基苯并[a]蒽的一种常见杂质在诱导形态学改变以及使鸡胚成纤维细胞的葡萄糖摄取、DNA合成和细胞数量增加方面比7,12-二甲基苯并[a]蒽更有效。当原代或传代培养物用3微克该化合物/毫升处理时，2天后DNA合成增加，随后逐渐发生形态学转变。通过氧化铝柱色谱从7,12-二甲基苯并[a]蒽中分离出的该化合物，通过薄层层析、质谱分析、碳氢分析、紫外光谱和核磁共振光谱以及热分解进行了表征。它是7,12-二甲基苯并[a]蒽的光氧化产物，7,12-环氧-7,12-二甲基苯并[a]蒽。有人提出，用7,12-二甲基苯并[a]蒽处理培养物后观察到的一些生物学效应可能部分归因于光氧化产物的存在。

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。

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METABOLISM OF POLYCYCLIC COMPOUNDS. THE METABOLISM OF 7,12-DIMETHYLBENZ(ALPHA)ANTHRACENE BY RAT-LIVER HOMOGENATES.多环化合物的代谢。大鼠肝脏匀浆对7,12-二甲基苯并（α）蒽的代谢

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In vitro malignant transformation of cells derid from mouse prostate in the presence of 3-methylcholanthrene.在存在3-甲基胆蒽的情况下，源自小鼠前列腺的细胞的体外恶性转化。（注：原文中“derid”有误，可能是“derived”）

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Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. II. Cellular responses during enzyme induction.哺乳动物细胞培养中底物诱导的微粒体芳基羟化酶。II. 酶诱导过程中的细胞反应。

J Biol Chem. 1968 Dec 10;243(23):6250-61.

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Effects of local cell concentrations upon the growth of chick embryo cells in tissue culture.局部细胞浓度对组织培养中鸡胚细胞生长的影响。

Exp Cell Res. 1968 Mar;49(3):666-78. doi: 10.1016/0014-4827(68)90213-9.

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Reactions between singlet oxygen and the constituents of nucleic acids. Importance of reactions in photodynamic processes.单线态氧与核酸成分之间的反应。光动力过程中反应的重要性。

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In vitro malignant transformation by methylcholanthrene of the progeny of single cells derived from C3H mouse prostate.用甲基胆蒽对源自C3H小鼠前列腺的单细胞后代进行体外恶性转化。

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10

Morphological, oncogenic, and karyological characteristics of Syrian hamster embryo cells transformed in vitro by carcinogenic polycyclic hydrocarbons.经致癌多环烃体外转化的叙利亚仓鼠胚胎细胞的形态学、致癌性和染色体特征

Cancer Res. 1971 Aug;31(8):1118-27.

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