Vijayaraghavan Renuka, Maruvajala Vidyavathi, Venkata Rayadurgam Suresh Kumar, Thomas Sabu, Loganathan Sravanthi, Valapa Ravi Babu
Electrochemical Process Engineering, Council of Scientific and Industrial Research (CSIR)-Central Electrochemical Research Institute (CECRI), Karaikudi 630003, Tamil Nadu, India.
Academy of Scientific and Innovative Research, Ghaziabad 201002, India.
ACS Appl Bio Mater. 2025 Sep 15;8(9):8084-8100. doi: 10.1021/acsabm.5c01068. Epub 2025 Aug 11.
There is still much need for improvement in the treatment of chronic full thickness wounds, which are among the most catastrophic injuries with direct consequences for public health systems. The purpose of this study is to investigate the influence of a poly(lactic acid) (PLA)-based scaffold containing silver nanoparticles (AgNPs) bound with cerium nitrate (CN) on fastening the recovery of wounds. A chemical reduction method employing trinyl citrate was used for the synthesis of AgNPs. Thioglycolic acid was used here to modify the surface of AgNPs, making them more stable and allowing them to attach to CN. The synthesized nanoparticles were analyzed for their physicochemical characteristics. The scaffolds were 3D-printed by using a pneumatic-extrusion-based process. Physical, thermal, antimicrobial, degradation, and cytocompatibility aspects of the constructed scaffolds were studied. The bioactivity, cell growth rate, and effectiveness as well as the method by which the 3D-printed scaffolds enhance skin regeneration were examined by both cell cultures and live animals using a model of complete skin damage. Histological analysis revealed that PLA/CN20-fAgNP scaffolds stimulated the formation of granulation tissue. Furthermore, the inclusion of CN20-fAgNP substantially improved regeneration of the wounded area after implantation in rat skin defects. The regenerated tissues had a dermal shape and composition that closely resembled that of healthy skin.
慢性全层伤口是对公共卫生系统有直接影响的最严重损伤之一，其治疗仍有很大的改进空间。本研究的目的是调查一种基于聚乳酸（PLA）的支架，该支架含有与硝酸铈（CN）结合的银纳米颗粒（AgNP）对加速伤口愈合的影响。采用柠檬酸三乙酯的化学还原法合成AgNP。在此使用巯基乙酸修饰AgNP的表面，使其更稳定并使其能够附着于CN。对合成的纳米颗粒进行了物理化学特性分析。通过基于气动挤压的工艺对支架进行3D打印。研究了构建支架的物理、热、抗菌、降解和细胞相容性方面。通过细胞培养和使用完全皮肤损伤模型的活体动物，研究了3D打印支架促进皮肤再生的生物活性、细胞生长速率和有效性以及方法。组织学分析表明，PLA/CN20-fAgNP支架刺激了肉芽组织的形成。此外，在大鼠皮肤缺损处植入后，CN20-fAgNP的加入显著改善了伤口区域的再生。再生组织具有与健康皮肤非常相似的真皮形状和组成。
