Zhong Jian, Li Ju, Liao Jichao, Ma Yanqin, Li Zhi, Yang Liang, Chang Wei, Miao Mingjun
Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province, Sichuan Academy of Agricultural Sciences, Horticulture Research Institute, Chengdu, China.
Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture and Rural Affairs, Chengdu, China.
Front Microbiol. 2025 Jul 28;16:1623052. doi: 10.3389/fmicb.2025.1623052. eCollection 2025.
Alpine agroecosystems present unique crop production challenges due to extreme environmental conditions, where rhizosphere microbiomes significantly influence plant adaptation.
To investigate mulch-induced microbial changes in high-altitude agriculture, this study analyzed a radish field in China using SMRT sequencing (16S rRNA/ITS) and metagenomics, comparing PBAT/PLA biodegradable films with/without humic acid (HA) at varying thicknesses.
Results demonstrated that radish cultivation selectively enriched and while depleting and , with fungal communities shifting from -to -dominance. Notably, HA-amended mulches enhanced bacterial diversity and specifically promoted polymer-degrading microbes (). Metagenomic profiling revealed thickness-dependent increases in functional genes related to carbohydrate and amino acid metabolism in HA-treated soils.
These findings advance our understanding of how biodegradable mulch formulations can be optimized to enhance microbial ecosystem services in alpine farming systems.
由于极端的环境条件，高山农业生态系统面临着独特的作物生产挑战，其中根际微生物群对植物适应性有显著影响。
为了研究地膜覆盖对高海拔农业中微生物的影响，本研究利用单分子实时测序技术（16S rRNA/ITS）和宏基因组学对中国的一块萝卜地进行了分析，比较了不同厚度的含腐殖酸（HA）和不含腐殖酸的聚己二酸/对苯二甲酸丁二醇酯/聚乳酸（PBAT/PLA）可生物降解地膜。
结果表明，萝卜种植选择性地富集了 而减少了 和 ，真菌群落从 优势转变为 优势。值得注意的是，添加HA的地膜提高了细菌多样性，并特别促进了聚合物降解微生物（ ）。宏基因组分析表明，在HA处理的土壤中，与碳水化合物和氨基酸代谢相关的功能基因的丰度随地膜厚度增加。
这些发现增进了我们对如何优化可生物降解地膜配方以增强高山农业系统中微生物生态系统服务的理解。
