Qian Ning, Wu Yuhong, Zhang Wei, Yang Jun, Bhadauria Vijai, Zhang Guozhen, Yan Jiye, Zhao Wensheng
MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, Department of Plant Biosecurity, China Agricultural University, Beijing 100193, China.
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
J Fungi (Basel). 2024 Nov 8;10(11):776. doi: 10.3390/jof10110776.
Cover crops, typically planted during off-seasons and requiring less agronomic manipulation, may provide abundant fungal resources. Certain species of could serve as potential agents for controlling plant diseases and developing bioorganic fertilizers. Eight species from five genera of were identified from healthy and , two major cover crops, through multigene phylogenetic analysis, morphological identification, and pairwise homoplasy index testing. The identified species comprise three new species: , , and , as well as five known but new host record species: , , , , and . Dual culture tests revealed that isolates of all eight species exhibited antagonistic effects on multiple phytopathogens. Among the identified fungi, the NSJA2 isolate, belonging to , exhibited significant relative inhibition effects on 14 out of 15 phytopathogens tested in this study, indicating its broad-spectrum antagonistic effects. Additionally, NSJA2 exhibited excellent salt tolerance. Overall, our study has identified multiple fungi with anti-phytopathogens potential, among which NSJA2 exhibits high potential for practical application. This finding paves the way for further exploration and exploitation of NSJA2 as a promising biocontrol agent.
覆盖作物通常在非生长季节种植，所需农艺操作较少，可能会提供丰富的真菌资源。某些种类的[此处原文缺失具体菌类名称]可作为控制植物病害和开发生物有机肥的潜在媒介。通过多基因系统发育分析、形态鉴定和成对同塑性指数测试，从两种主要覆盖作物——[此处原文缺失具体作物名称1]和[此处原文缺失具体作物名称2]的健康植株中鉴定出了来自5个属的8个[此处原文缺失具体菌类名称]物种。鉴定出的物种包括3个新物种：[此处原文缺失具体新物种名称1]、[此处原文缺失具体新物种名称2]和[此处原文缺失具体新物种名称3]，以及5个已知但有新寄主记录的物种：[此处原文缺失具体已知物种名称1]、[此处原文缺失具体已知物种名称2]、[此处原文缺失具体已知物种名称3]、[此处原文缺失具体已知物种名称4]和[此处原文缺失具体已知物种名称5]。对峙培养试验表明，所有8个[此处原文缺失具体菌类名称]物种的分离株对多种植物病原菌均表现出拮抗作用。在鉴定出的真菌中，属于[此处原文缺失具体菌类属名]的NSJA2分离株对本研究中测试的15种植物病原菌中的14种表现出显著的相对抑制作用，表明其具有广谱拮抗作用。此外，NSJA2表现出优异的耐盐性。总体而言，我们的研究鉴定出了多种具有抗植物病原菌潜力的真菌，其中NSJA2具有很高的实际应用潜力。这一发现为进一步探索和开发NSJA2作为一种有前景的生物防治剂铺平了道路。
