工程化富含sn-2 DHA的三酰甘油：脂肪酶催化合成与溶剂分级分离的比较

Engineered sn-2 DHA-enriched triacylglycerols: Comparison of lipase-catalyzed synthesis and solvent fractionation.

作者信息

Li Feng, Zhang Yanan, Wang Yue, Wang Song, Wang Xingguo, Wei Wei

机构信息

State Key Lab of Food Science and Resources, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Food Laboratory of Zhongyuan, Luohe 462300, China.

出版信息

Food Chem. 2025 Nov 30;493(Pt 2):145855. doi: 10.1016/j.foodchem.2025.145855. Epub 2025 Aug 7.

DOI:10.1016/j.foodchem.2025.145855

PMID:40795564

Abstract

Sn-2 DHA-enriched triacylglycerols (TAG) were prepared by both lipase-catalyzed esterification and solvent fractionation. The specific-structured triacylglycerol (SST) was synthesized at a molar ratio of algae oil to mixed oleic acid and linoleic acid (3:2, w/w) of 1:5 with 8 % of Lipozyme TL IM at 60 °C for 8 h. In the SSTs, the DHA content reached 46 % with sn-2 DHA/total DHA of 50 %. The optimal fractionation conditions were algae oil to solvent (v/v) of 1:6 in acetone (-20 °C) for 6 h. DHA in the final fraction was 37 % and sn-2 DHA/total DHA was 60 %. LC-MS analysis confirmed the TAG profiles, showing that SSTs predominantly contained U-DHA-U type TAGs (U, unsaturated fatty acid), whereas the fractionated solids were enriched in S-DHA-S type TAGs (S, saturated fatty acid). The melting points of the final products were below the physiological temperature (36.6-37.3 °C), enabling efficient absorption and utilization by the human body.

摘要

富含Sn-2 DHA的三酰甘油（TAG）通过脂肪酶催化酯化和溶剂分级分离两种方法制备。在60℃下，以1:5的藻油与混合油酸和亚油酸（3:2，w/w）的摩尔比，加入8%的Lipozyme TL IM合成特定结构的三酰甘油（SST），反应8小时。在SST中，DHA含量达到46%，Sn-2 DHA/总DHA为50%。最佳分级分离条件为在丙酮（-20℃）中藻油与溶剂的比例为1:6（v/v），分离6小时。最终馏分中的DHA为37%，Sn-2 DHA/总DHA为60%。LC-MS分析证实了TAG谱图，表明SST主要含有U-DHA-U型TAG（U，不饱和脂肪酸），而分级分离得到的固体富含S-DHA-S型TAG（S，饱和脂肪酸）。最终产品的熔点低于生理温度（36.6-37.3℃），有利于人体的有效吸收和利用。