Rabello-Gay M N, Carvalho M I, Otto P A, Targa H J
Mutat Res. 1985 Dec;158(3):181-8. doi: 10.1016/0165-1218(85)90082-5.
7-week-old and 12-week-old mice of both sexes received either a control or protein-deficient diet for 3 weeks. Afterwards, they were given a single dose of cyclophosphamide (0.5 mg/10 g b.wt.) before being sacrificed. The relationship between age and the clastogenic action of cyclophosphamide can be observed in the bone marrow cells of male mice but not in those of female mice. 12-week-old males on a 75% protein-deficient diet have a lower frequency of cells with cyclophosphamide-induced chromosome aberrations than has the control group. On the contrary, 7-week-old males and females, and 12-week-old females, show that protein-deficient diets act synergistically with the clastogenic action of cyclophosphamide. These results are discussed taking the metabolism of the drug into account. Animal age also plays a role in the formation of chromosome rearrangements; this type of aberration is significantly more frequent in younger animals of both sexes than in older ones exposed to the drug.
对7周龄和12周龄的雌雄小鼠均给予对照饮食或蛋白质缺乏饮食，持续3周。之后，在处死前给它们单次注射环磷酰胺（0.5毫克/10克体重）。在雄性小鼠的骨髓细胞中可观察到年龄与环磷酰胺致断裂作用之间的关系，而在雌性小鼠中则未观察到。饮食中蛋白质含量为75%缺乏的12周龄雄性小鼠，其环磷酰胺诱导的染色体畸变细胞频率低于对照组。相反，7周龄的雄性和雌性小鼠以及12周龄的雌性小鼠显示，蛋白质缺乏饮食与环磷酰胺的致断裂作用具有协同作用。结合药物代谢对这些结果进行了讨论。动物年龄在染色体重排的形成中也起作用；在接触该药物的两性幼龄动物中，这种类型的畸变明显比老龄动物更频繁。
