Schneider M, Darlix J L, Erickson J, Rochaix J D
Nucleic Acids Res. 1985 Dec 9;13(23):8531-41. doi: 10.1093/nar/13.23.8531.
The flanking regions and the end of the chloroplast ribosomal unit of Chlamydomonas reinhardii have been sequenced. The upstream region of the ribosomal unit contains three open reading frames coding for 111, 117 and 124 amino acids, respectively. The latter polypeptide is partially related to the ribosomal protein L16 of E. coli. Two of the open reading frames overlap each other and are oriented in opposite direction. The region between these open reading frames and the 5' end of the 16S rRNA gene contains numerous short direct and inverted repeats which can be folded into large stem-loop structures. Sequence elements that resemble prokaryotic promoters are found in the same region. Several of the repeated elements are distributed throughout the non-coding regions of the chloroplast inverted repeat. Sequence comparison between the 5S rRNA and its gene does not reveal any significant sequence heterogeneity between the chloroplast 5S rRNA genes.
莱茵衣藻叶绿体核糖体单元的侧翼区域和末端已被测序。核糖体单元的上游区域包含三个开放阅读框，分别编码111、117和124个氨基酸。后一种多肽与大肠杆菌的核糖体蛋白L16部分相关。其中两个开放阅读框相互重叠且方向相反。这些开放阅读框与16S rRNA基因5'端之间的区域包含许多短的正向和反向重复序列，这些序列可折叠成大的茎环结构。在同一区域发现了类似于原核启动子的序列元件。几个重复元件分布在叶绿体反向重复序列的非编码区域。5S rRNA与其基因之间的序列比较未揭示叶绿体5S rRNA基因之间存在任何显著的序列异质性。
