Thongkhao Kannika, Intharuksa Aekkhaluck, Phrutivorapongkul Ampai
School of Languages and General Education, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Herbology Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Int J Mol Sci. 2025 Jul 24;26(15):7168. doi: 10.3390/ijms26157168.
The root of L. is commercially available in herbal markets in both crude and powdered forms. root is a key ingredient in numerous polyherbal formulations. However, has two closely related species, L. and Lam. Since only is traditionally used in Thai polyherbal products, adulteration with other species could potentially compromise the therapeutic efficacy and overall effectiveness of these formulations. To address this issue, a MassARRAY iPLEX assay was developed to accurately identify and differentiate from its closely related species. Five single nucleotide polymorphism (SNP) sites-positions 18, 112, 577, 623, and 652-within the internal transcribed spacer (ITS) region were selected as genetic markers for species identification. The assay demonstrated high accuracy in identifying and was capable of detecting the species at DNA concentrations as low as 0.01 ng/µL. Additionally, the assay successfully identified in commercial crude drug samples, highlighting potential instances of adulteration. Furthermore, it was able to distinguish in mixed samples containing along with either or . The developed MassARRAY iPLEX assay proves to be a reliable and effective molecular tool for authenticating raw materials. Its application holds significant potential for ensuring the integrity of herbal products by preventing misidentification and adulteration.
L.的根在草药市场上有粗品和粉末两种商业形式。其根是众多复方草药制剂中的关键成分。然而，L.有两个近缘物种，即L.和Lam.。由于传统上只有L.用于泰国复方草药产品，与其他物种的掺假可能会损害这些制剂的治疗效果和整体功效。为了解决这个问题，开发了一种MassARRAY iPLEX检测方法，以准确识别L.并将其与其近缘物种区分开来。选择内部转录间隔区（ITS）区域内的五个单核苷酸多态性（SNP）位点——第18、112、577、623和652位——作为物种鉴定的遗传标记。该检测方法在识别L.方面显示出高准确性，并且能够在低至0.01 ng/µL的DNA浓度下检测到该物种。此外，该检测方法成功地在商业粗药样品中鉴定出L.，突出了潜在的掺假情况。此外，它能够在含有L.以及L.或Lam.的混合样品中区分出L.。所开发的MassARRAY iPLEX检测方法被证明是一种可靠且有效的分子工具，用于鉴定L.的原材料。其应用在通过防止误认和掺假来确保草药产品的完整性方面具有巨大潜力。
