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不同溶剂中（L.）F.H. 维格干提取物的抗氧化和细胞毒性活性

Antioxidant and Cytotoxic Activities of (L.) F.H. Wigg. Dry Extracts in Different Solvents.

作者信息

Popovici Violeta, Bucur Laura, Popescu Antoanela, Schröder Verginica, Costache Teodor, Rambu Dan, Cucolea Iulia Elena, Gîrd Cerasela Elena, Caraiane Aureliana, Gherghel Daniela, Vochita Gabriela, Badea Victoria

机构信息

Department of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, Romania.

Department of Pharmacognosy, Faculty of Pharmacy, Ovidius University of Constanta, 6 Capitan Al. Serbanescu Street, 900001 Constanta, Romania.

出版信息

Plants (Basel). 2021 May 1;10(5):909. doi: 10.3390/plants10050909.

DOI:10.3390/plants10050909
PMID:34062835
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8147331/
Abstract

Lichens represent a significant source of antioxidants due to numerous metabolites that can reduce free radicals. (L.) F.H. Wigg. has been recognized and used since ancient times for its therapeutic effects, some of which are based on its antioxidant properties. The present study aims to analyze the phytochemical profile and to evaluate the antioxidant and cytotoxic potential of this lichen species. Five dry extracts of (UBDE) in different solvents (acetone, ethyl acetate, ethanol, methanol, water) were prepared by refluxing at Soxhlet to achieve these proposed objectives and to identify which solvent is the most effective for the extraction. The usnic acid content (UAC) was quantified by ultra-high performance liquid chromatography (UHPLC). The total polyphenols content (TPC) and tannins content (TC) were evaluated by spectrophotometry, and the total polysaccharides (PSC) were extracted by a gravimetric method. The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical method was used to assess the antioxidant activity (AA) and the Brine Shrimp Lethality (BSL) assay was the biotest for cytotoxic activity evaluation. The ethyl acetate extract had the highest usnic acid content, and acetone extract had the highest content of total polyphenols and tannins. The most significant antioxidant effect was reported to methanol extract, and all the extracts proved high cytotoxicity. The water extract has the lowest cytotoxicity because usnic acid is slightly soluble in this solvent, and it was not found at UHPLC analysis. All extracts recorded a moderate correlation between the content of usnic acid, polyphenols, tannins, and AA; furthermore, it has been observed that the cytotoxicity varies inversely with the antioxidant effect.

摘要

地衣由于含有多种能够减少自由基的代谢产物，是抗氧化剂的重要来源。地卷（L.）F.H. Wigg. 自古以来就因其治疗作用而被认可和使用，其中一些作用基于其抗氧化特性。本研究旨在分析这种地衣物种的植物化学特征，并评估其抗氧化和细胞毒性潜力。通过索氏回流法制备了地卷（UBDE）在不同溶剂（丙酮、乙酸乙酯、乙醇、甲醇、水）中的五种干提取物，以实现这些既定目标，并确定哪种溶剂对提取最有效。通过超高效液相色谱（UHPLC）对地衣酸含量（UAC）进行定量。通过分光光度法评估总多酚含量（TPC）和单宁含量（TC），并通过重量法提取总多糖（PSC）。采用2,2-二苯基-1-苦基肼基水合物（DPPH）自由基法评估抗氧化活性（AA），采用卤虫致死率（BSL）试验作为评估细胞毒性活性的生物测试。乙酸乙酯提取物的地衣酸含量最高，丙酮提取物的总多酚和单宁含量最高。据报道，甲醇提取物的抗氧化效果最显著，并且所有提取物都具有高细胞毒性。水提取物的细胞毒性最低，因为地衣酸在该溶剂中微溶，并且在UHPLC分析中未检测到。所有提取物在地衣酸、多酚、单宁和AA含量之间呈现出中等相关性；此外，还观察到细胞毒性与抗氧化效果呈反比。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/1d82b4e48d35/plants-10-00909-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/1980f8eaf80e/plants-10-00909-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/b922c4dbe2f8/plants-10-00909-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/f206194cbec8/plants-10-00909-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/acf45743fca9/plants-10-00909-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/fc290bafcbe6/plants-10-00909-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/1d82b4e48d35/plants-10-00909-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/1980f8eaf80e/plants-10-00909-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/b922c4dbe2f8/plants-10-00909-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/f206194cbec8/plants-10-00909-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/acf45743fca9/plants-10-00909-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/fc290bafcbe6/plants-10-00909-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34fe/8147331/1d82b4e48d35/plants-10-00909-g006.jpg

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2
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Antioxidants (Basel). 2020 Sep 14;9(9):864. doi: 10.3390/antiox9090864.
3
Diet and Mental Health: Review of the Recent Updates on Molecular Mechanisms.饮食与心理健康：分子机制的最新进展综述
Melinjo Seeds ( L.) Antioxidant Activity and Cytotoxic Effects on MCF-7 Breast Cancer Cells: A Study Based on Tracing of Resveratrol Compound.
美藤果种子（L.）对MCF-7乳腺癌细胞的抗氧化活性和细胞毒性作用：基于白藜芦醇化合物追踪的研究
J Pharm Bioallied Sci. 2024 Jan-Mar;16(1):16-23. doi: 10.4103/jpbs.jpbs_878_23. Epub 2024 Mar 21.
4
Anti-viral effect of usenamine a using SARS-CoV-2 pseudo-typed viruses.乌司他丁胺A对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）假型病毒的抗病毒作用。
Heliyon. 2023 Oct 28;9(11):e21742. doi: 10.1016/j.heliyon.2023.e21742. eCollection 2023 Nov.
5
(Jacq) Bory: Metabolites and Biological Activities.雅格·博里：代谢产物和生物活性。
Molecules. 2023 Oct 28;28(21):7317. doi: 10.3390/molecules28217317.
6
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Antioxidants (Basel). 2022 Aug 19;11(8):1601. doi: 10.3390/antiox11081601.
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4
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5
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Int J Mol Sci. 2020 Jan 29;21(3):876. doi: 10.3390/ijms21030876.
6
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7
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8
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