Continuous mechanical pressure and joint tissue. Effect of synovial membrane products and indomethacin in vitro.

We have previously reported that a continuous mechanical pressure of approximately 30 kgfcm-2 greatly inhibited the degradation of articular proteoglycans in calves. In the present report it is shown that indomethacin (5 micrograms/ml) failed to inhibit cartilage degradation caused by mechanical pressure. It is therefore suggested that prostaglandins do not mediate the inhibition of cartilage degradation exerted by continuous mechanical pressure. We have also reported previously that bovine-conditioned synovial medium (SM) greatly enhances the degradation of calf articular cartilage proteoglycans. We now show that the catabolic activity of SM was greatly (but not entirely) inhibited by the application of a continuous mechanical pressure. Simultaneous addition of indomethacin (5 micrograms/ml) to the cartilage cultures did not affect this result. However, the addition of indo-SM (SM from synovial tissue cultured in the presence of 5 micrograms/ml indomethacin) to cartilage cultures subjected to continuous mechanical pressure did not increase cartilage degradation, compared with the addition of DMEM alone. A hypothesis is proposed that continuous mechanical pressure may cause a change in the state of synovial factor receptors on the membranes of chondrocytes.