Klämfeldt A
Scand J Rheumatol. 1985;14(4):431-7. doi: 10.3109/03009748509102050.
We have previously reported that a continuous mechanical pressure of approximately 30 kgfcm-2 greatly inhibited the degradation of articular proteoglycans in calves. In the present report it is shown that indomethacin (5 micrograms/ml) failed to inhibit cartilage degradation caused by mechanical pressure. It is therefore suggested that prostaglandins do not mediate the inhibition of cartilage degradation exerted by continuous mechanical pressure. We have also reported previously that bovine-conditioned synovial medium (SM) greatly enhances the degradation of calf articular cartilage proteoglycans. We now show that the catabolic activity of SM was greatly (but not entirely) inhibited by the application of a continuous mechanical pressure. Simultaneous addition of indomethacin (5 micrograms/ml) to the cartilage cultures did not affect this result. However, the addition of indo-SM (SM from synovial tissue cultured in the presence of 5 micrograms/ml indomethacin) to cartilage cultures subjected to continuous mechanical pressure did not increase cartilage degradation, compared with the addition of DMEM alone. A hypothesis is proposed that continuous mechanical pressure may cause a change in the state of synovial factor receptors on the membranes of chondrocytes.
我们之前曾报道，约30 kgf/cm²的持续机械压力能极大地抑制小牛关节蛋白聚糖的降解。在本报告中，结果显示吲哚美辛（5微克/毫升）未能抑制由机械压力引起的软骨降解。因此，提示前列腺素并不介导持续机械压力对软骨降解的抑制作用。我们之前还报道过，牛条件培养液（SM）能极大地增强小牛关节软骨蛋白聚糖的降解。我们现在表明，持续机械压力的施加能极大地（但并非完全）抑制SM的分解代谢活性。向软骨培养物中同时添加吲哚美辛（5微克/毫升）并不影响这一结果。然而，与仅添加杜氏改良伊格尔培养基（DMEM）相比，向经受持续机械压力的软骨培养物中添加吲哚美辛培养液（在5微克/毫升吲哚美辛存在下培养的滑膜组织培养液）并不会增加软骨降解。提出了一个假说，即持续机械压力可能会导致软骨细胞膜上滑膜因子受体状态的改变。
