Mazloomi Sahar, Barartabar Zeinab, Pilehvari Shamim
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Students Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran.
J Reprod Infertil. 2023 Jan-Mar;24(1):26-34. doi: 10.18502/jri.v24i1.11906.
There is an association between inflammatory factors and polycystic ovary syndrome (PCOS) and most of women with PCOS experience the symptoms of hirsutism. The purpose of this study was to evaluate the role of obesity in PCOS occurrence, which is linked with inflammation and hirsutism.
This study was designed as a case-control research. It was performed on 102 women with PCOS and 102 healthy women as controls who were age-matched. Serum concentrations of testosterone, estradiol (E2), IL-1, IL-6, high-sensitivity c-reactive protein (hs-CRP), and aromatase activity were measured in blood samples. Statistical tests including unpaired t-tests, Mann-Whitney U test, Kruskal-Wallis, Spearman's correlation, and Chi-square tests were used for data analysis. Statistical significance was set at p<0.05.
A significant difference was found between hs-CRP, IL-1, and IL-6 in PCOS patients and healthy individuals (p<0.001). Aromatase activity was markedly lower in PCOS cases. The serum level of IL-1 (p=0.392) and IL-6 (p=0.764) was not different between overweight and normal weight women. In both studied groups (case and control), hirsutism frequency was markedly higher in individuals with BMI ≥25 (p<0.05). Inflammatory factors significantly affected the PCOS group (p<0.05). However, logistic regression showed that hs-CRP increment is more effective on increasing the risk of PCOS (OR: 6.324, p<0.001).
In this study, hs-CRP, IL-1, and IL-6 levels increased in all PCOS women Although the incidence of hirsutism in PCOS is associated with obesity, in PCOS pathogenesis, only IL-1 and IL-6 were independent of BMI.
炎症因子与多囊卵巢综合征（PCOS）之间存在关联，且大多数PCOS女性会出现多毛症状。本研究的目的是评估肥胖在PCOS发生中的作用，肥胖与炎症和多毛症相关。
本研究设计为病例对照研究。对102例PCOS女性和102例年龄匹配的健康女性作为对照进行研究。检测血样中睾酮、雌二醇（E2）、白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、高敏C反应蛋白（hs-CRP）的血清浓度以及芳香化酶活性。数据分析采用包括非配对t检验、曼-惠特尼U检验、克鲁斯卡尔-沃利斯检验、斯皮尔曼相关性检验和卡方检验等统计方法。设定统计学显著性为p<0.05。
PCOS患者与健康个体之间hs-CRP、IL-1和IL-6存在显著差异（p<0.001）。PCOS病例中芳香化酶活性明显较低。超重和正常体重女性之间IL-1（p=0.392）和IL-6（p=0.764）的血清水平无差异。在两个研究组（病例组和对照组）中，BMI≥25的个体多毛症发生率明显更高（p<0.05）。炎症因子对PCOS组有显著影响（p<0.05）。然而，逻辑回归显示hs-CRP升高对增加PCOS风险更有效（比值比：6.324，p<0.001）。
在本研究中，所有PCOS女性的hs-CRP、IL-1和IL-6水平均升高。虽然PCOS中多毛症的发生率与肥胖有关，但在PCOS发病机制中，只有IL-1和IL-6与BMI无关。
