Lallow Emran O, Busha Kishankumar J, Park Sarah H, Atzampou Maria, Jhumur Nandita C, Demiryurek Yasir, Roberts Christine C, Shan Jerry W, Zahn Jeffrey D, Shreiber David I, Park Young K, Singer Jonathan P, Maslow Joel N, Lin Hao
Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, United States.
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, United States.
Front Drug Deliv. 2023 Jan 13;3:1095181. doi: 10.3389/fddev.2023.1095181. eCollection 2023.
Intradermal (ID) injection is a technique widely used in laboratorial and clinical applications. The boundary of the dome-like bleb formed during injection is assumed to represent the lateral extent of the injected material. This work systematically characterizes cargo molecule distribution (puddle) as a function of injection volume and molecular/particle size in rat skin post ID injection. In general, results indicate that the puddle forms a subdomain laterally contained within the bleb, with an area inversely correlating to the molecular size of the injected material. For 50 μL and 100 µL injections, the average area of the bleb was 40.97 ± 6.30 mm and 55.64 ± 8.20 mm, respectively, regardless of the molecular/particle size. On the other hand, the area of the puddle was dependent on the molecular size and ranged between 45.38 ± 8.29 mm and 6.14 ± 4.50 mm for 50 µL injections, and 66.64 ± 11.22 mm and 11.50 ± 9.67 mm for 100 µL injections. The lateral distribution appears to have no time-dependency up to 10 min post injection. The trend in the depth of cargo penetration is also similar, with smaller particles extending deeper into the dermis and subcutaneous fat layers. Because the area of puddle can be significantly less than that of the bleb, establishing base characterization is essential to understand cellular interactions with the injected biological substances.
皮内注射（ID）是一种在实验室和临床应用中广泛使用的技术。注射过程中形成的圆顶状水疱的边界被认为代表了注射物质的横向范围。这项工作系统地描述了大鼠皮内注射后，作为注射体积和分子/颗粒大小函数的货物分子分布（液池）情况。一般来说，结果表明液池在水疱横向范围内形成一个子区域，其面积与注射物质的分子大小呈负相关。对于50μL和100μL的注射量，无论分子/颗粒大小如何，水疱的平均面积分别为40.97±6.30平方毫米和55.64±8.20平方毫米。另一方面，液池的面积取决于分子大小，50μL注射量时其面积在45.38±8.29平方毫米至6.14±4.50平方毫米之间，100μL注射量时在66.64±11.22平方毫米至11.50±9.67平方毫米之间。注射后10分钟内，横向分布似乎没有时间依赖性。货物渗透深度的趋势也类似，较小的颗粒延伸到真皮和皮下脂肪层的深度更深。由于液池的面积可能明显小于水疱的面积，建立基础特征对于理解细胞与注射生物物质的相互作用至关重要。
