Jeong Inae, Lee Juhwan, Park Soo-Jeung, Kim Ok-Kyung
Division of Food and Nutrition, Chonnam National University, Gwangju, 61186, Republic of Korea.
Department of Culinary and Nutrition, Woo-song University, Daejeon, 34606, Republic of Korea.
Sci Rep. 2025 Aug 23;15(1):31010. doi: 10.1038/s41598-025-15594-5.
Obesity is closely related to liver disease. However, few studies have focused on the impact of adipose tissue-derived extracellular vesicles (EVs) in obesity on liver disease. Therefore, we aimed to investigate the effect of adipose tissue-derived EVs from high-fat diet (HFD)-fed obese mice (EV-HFD) on liver damage induced by oxidative stress. We investigated alterations in the expression of antioxidant enzymes in adipose tissue, and the loading of those enzymes into adipose tissue-derived EVs. Furthermore, we treated alpha mouse liver 12 (AML12) cells with adipose tissue-derived EVs and induced oxidative stress. We observed that the HFD did not exert an effect on the protein expressions of antioxidant enzymes in adipose tissue. Intriguingly, the EV-HFD exhibited an upregulation in the loading of catalase (CAT) when compared to the adipose tissue-derived EVs from normal chow-fed mice (EV-NC). Notably, both types of EVs exhibited a similar capacity to mitigate cell damage when exposed to oxidative stress. Our findings indicate that obesity-induced loading of more CAT into adipose tissue-derived EVs cannot improve their antioxidant capacity in AML12 cells. We suggest that adipose tissue-derived EVs can serve as a tool to maintain homeostasis and defend against oxidative stress, thereby supporting normal physiological functions.
肥胖与肝脏疾病密切相关。然而，很少有研究关注肥胖状态下脂肪组织衍生的细胞外囊泡（EVs）对肝脏疾病的影响。因此，我们旨在研究高脂饮食（HFD）喂养的肥胖小鼠脂肪组织衍生的EVs（EV-HFD）对氧化应激诱导的肝损伤的影响。我们研究了脂肪组织中抗氧化酶表达的变化，以及这些酶在脂肪组织衍生的EVs中的装载情况。此外，我们用脂肪组织衍生的EVs处理α小鼠肝脏12（AML12）细胞并诱导氧化应激。我们观察到高脂饮食对脂肪组织中抗氧化酶的蛋白表达没有影响。有趣的是，与正常饮食喂养小鼠的脂肪组织衍生的EVs（EV-NC）相比，EV-HFD中过氧化氢酶（CAT）的装载量上调。值得注意的是，当暴露于氧化应激时，两种类型的EVs减轻细胞损伤的能力相似。我们的研究结果表明，肥胖诱导更多的CAT装载到脂肪组织衍生的EVs中并不能提高其在AML12细胞中的抗氧化能力。我们认为脂肪组织衍生的EVs可以作为维持体内稳态和抵御氧化应激的工具，从而支持正常的生理功能。
