Zhang Yuli, Liu Yujie, Liu Lin, Tu Yingxin, Ren Meixi, Niu Guoyu, Sun Hengyi, Zhu Yujing
Central Sterile Supply Department, Affiliated Hospital of Shandong Second Medical University, Weifang, 261031, China.
Immune-Path Biotechnology (Suzhou) Co., Ltd, Suzhou, 215000, China.
Sci Rep. 2025 Aug 26;15(1):31356. doi: 10.1038/s41598-025-16304-x.
Mosquitoes are important vectors of a wide range of viruses and serve as pivotal vectors with complex ecological functions that facilitate the transmission of these pathogens between hosts, such as humans, through biting behavior. In the mosquito surveillance conducted in Weifang and Yantai cities of Shandong Province, genetic material of a novel virus, temporarily named Fangshan bunya-like virus, was detected. From 3013 female mosquitoes, genetic material was detected in 9 pools (infection rate: 0.30%) using qRT-PCR and nested PCR. Analysis of the distances between pairs revealed a significant level of nucleotide similarity among these amplified sequences. Phylogenetic analysis placed Fangshan bunya-like virus within the Mobuvirus genus, Phenuiviridae family, clustering with Pyongtaek Culex Bunyavirus and indicating a common ancestor. The genomic characteristics and phylogenetic classification of Fangshan bunya-like virus offered valuable insights into the diversity and evolution of Phenuiviridae among mosquito populations, despite the challenge of not having isolated the live virus.
蚊子是多种病毒的重要传播媒介，作为具有复杂生态功能的关键媒介，通过叮咬行为促进这些病原体在人类等宿主之间传播。在山东省潍坊市和烟台市进行的蚊子监测中，检测到一种新型病毒的遗传物质，暂命名为房山布尼亚样病毒。在3013只雌蚊中，使用qRT-PCR和巢式PCR在9个样本池中检测到遗传物质（感染率：0.30%）。对序列对之间距离的分析表明，这些扩增序列之间存在显著水平的核苷酸相似性。系统发育分析将房山布尼亚样病毒置于布尼亚病毒科莫布病毒属内，与平泽库蚊布尼亚病毒聚类，表明有共同的祖先。尽管尚未分离出活病毒，但房山布尼亚样病毒的基因组特征和系统发育分类为了解蚊群中布尼亚病毒科的多样性和进化提供了有价值的见解。
