Jie Weiguang, Zhang Min, Tan Yiwen, Yang Haobo, Wang Wenkai, Kan Lianbao
Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region, Engineering Research Center of Agricultural Microbiology Technology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, China.
Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, China.
Front Plant Sci. 2025 Aug 8;16:1585035. doi: 10.3389/fpls.2025.1585035. eCollection 2025.
As global agriculture shifts toward an ecological civilization model, microbial fertilizers have emerged as a new strategy to promote plant growth and enhance soil fertility. In this study, the effects of and on soybean biomass, root rot disease index, chlorothalonil residue in soybean rhizosphere soil and grains, and the composition of the bacterial community in the rhizosphere soil were investigated through pot experiments. Soybean biomass, chlorothalonil residue and bacterial community were analyzed by direct measurement method, gas chromatographic method and high throughput sequencing, respectively. The findings demonstrated that in the and inoculation group, AMF spore density and colonization rate increased by 116.42% and 139.17%, respectively, compared to the control group. Microbial inoculum significantly enhanced the soybean biomass. Compared with the control group, the hundred-grain weight in the and inoculation group increased by 35.46%. The disease index of soybean root rot decreased by 77.78% in the and inoculation group relative to the control group. Furthermore, compared with the chlorothalonil-treated group, the chlorothalonil residue in both the rhizosphere soil and soybean grains in the chlorothalonil-treated and inoculated with and group decreased by 80.02% and 81.65%, respectively. Additionally, microbial inoculum and chlorothalonil application exerted substantial effects on the composition of the bacterial community. Specifically, co-inoculation with and led to an increase in the relative abundance of Acidobacteriota and Patescibacteria in the rhizosphere soil. Conversely, chlorothalonil application resulted in a reduction in the relative abundance of these bacterial taxa. The primary objective of this study was to provide theoretical support for the application of microbial inoculum as a strategy to mitigate soybean root rot, enhance growth, and reduce pesticide residue, thereby contributing to sustainable agricultural practices.
随着全球农业向生态文明模式转变，微生物肥料已成为促进植物生长和提高土壤肥力的新策略。本研究通过盆栽试验，研究了[具体微生物名称1]和[具体微生物名称2]对大豆生物量、根腐病病情指数、大豆根际土壤和籽粒中百菌清残留量以及根际土壤细菌群落组成的影响。分别采用直接测量法、气相色谱法和高通量测序法对大豆生物量、百菌清残留量和细菌群落进行了分析。结果表明，与对照组相比，[具体微生物名称1]和[具体微生物名称2]接种组的丛枝菌根真菌（AMF）孢子密度和定殖率分别提高了116.42%和139.17%。微生物接种剂显著提高了大豆生物量。与对照组相比，[具体微生物名称1]和[具体微生物名称2]接种组的百粒重增加了35.46%。[具体微生物名称1]和[具体微生物名称2]接种组的大豆根腐病病情指数相对于对照组降低了77.78%。此外，与百菌清处理组相比，百菌清处理并接种[具体微生物名称1]和[具体微生物名称2]组的根际土壤和大豆籽粒中的百菌清残留量分别降低了80.02%和81.65%。此外，微生物接种剂和百菌清的施用对细菌群落组成有显著影响。具体而言，[具体微生物名称1]和[具体微生物名称2]共同接种导致根际土壤中酸杆菌门和Patescibacteria的相对丰度增加。相反，施用百菌清导致这些细菌类群的相对丰度降低。本研究的主要目的是为微生物接种剂作为减轻大豆根腐病、促进生长和减少农药残留的策略的应用提供理论支持，从而有助于可持续农业实践。
