Schönmehl Rebecca, Winter Lina, Mendelsohn Daniel H, Cheung Wing-Hoi, Wong Ronald Man Yeung, Pabel Steffen, Sossalla Samuel, Brochhausen Christoph
Institute of Pathology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
Department of Neurology, LMU University Hospital, LMU Munich, 81377 Munich, Germany.
Methods Protoc. 2025 Aug 2;8(4):87. doi: 10.3390/mps8040087.
Mitochondria play a crucial role in adapting to fluctuating energy demands, particularly in various heart diseases. In addition to functional analyses such as the measurement of ROS or ATP, analysis of mitochondrial ultrastructure can be used to draw further conclusions about their functions and effects in tissue. In this protocol, we introduce a set of measurements to compare the ultrastructural and functional characteristics of human left ventricular mitochondria, using transmission electron microscopy (TEM). Measured parameters included mean size in µm, elongation, count, percental mitochondrial area in the measuring frame, and a conglomeration score. We also introduce a novel method of defining hydropic mitochondria as a comparable evaluation standard. With this cluster of measurement parameters, we aim to contribute a protocol for studying human mitochondrial morphology, distribution, and functionality.
线粒体在适应能量需求波动方面发挥着关键作用，尤其是在各种心脏疾病中。除了进行诸如活性氧（ROS）或三磷酸腺苷（ATP）测量等功能分析外，线粒体超微结构分析可用于进一步推断其在组织中的功能和作用。在本方案中，我们介绍了一组测量方法，使用透射电子显微镜（TEM）来比较人类左心室线粒体的超微结构和功能特征。测量参数包括以微米为单位的平均大小、伸长率、数量、测量框架中线粒体面积的百分比以及聚集分数。我们还引入了一种将水肿性线粒体定义为可比评估标准的新方法。通过这组测量参数，我们旨在提供一个研究人类线粒体形态、分布和功能的方案。
