Motta Chiara Maria, Fogliano Chiara, Trifuoggi Marco, Toscanesi Maria, Raggio Anja, Di Marino Simona, Venditti Paola, Fasciolo Gianluca, Avallone Bice, Carotenuto Rosa
Department of Biology, University of Naples Federico II, 80126 Naples, Italy.
Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy.
Toxics. 2025 Aug 20;13(8):700. doi: 10.3390/toxics13080700.
Polystyrene microfragments are among the most common plastic pollutants globally. They significantly affect aquatic life, harming various organs and tissues. In this study, we examined the effects of 3 µm polystyrene beads (MPs, 20 µg/L) on development and yolk resorption in pre-feeding nauplii of , a lecithotrophic crustacean used in toxicity testing. Results showed a reduced hatching rate, slower growth, and the onset of oxidative stress. Histological analysis revealed no significant morphological alteration; however, yolk platelets lost N-acetyl galactosamine (galNAc), and resorption was delayed. Lectin staining also showed a reduction in N-acetyl glucosamine (glcNAc) in the gut brush border, indicating impaired gut function. Gas chromatography detected the release of nanogram amounts of toxic volatile compounds (VOCs, ethylbenzene, xylene, benzaldehyde, and styrene) into the culture medium. In conclusion, the data demonstrate a delay in larval yolk resorption that can likely be attributed to the release of VOCs, which induce oxidative stress. Further research is urgently needed, given the potential biological and ecological implications of this finding.
聚苯乙烯微碎片是全球最常见的塑料污染物之一。它们对水生生物有显著影响，会损害各种器官和组织。在本研究中，我们检测了3微米聚苯乙烯珠（微塑料，20微克/升）对一种用于毒性测试的卵黄营养型甲壳动物无节幼体摄食前发育和卵黄吸收的影响。结果显示孵化率降低、生长缓慢以及氧化应激的出现。组织学分析未发现明显的形态改变；然而，卵黄小板失去了N-乙酰半乳糖胺（galNAc），并且吸收延迟。凝集素染色还显示肠道刷状缘中的N-乙酰葡糖胺（glcNAc）减少，表明肠道功能受损。气相色谱法检测到纳克量的有毒挥发性化合物（VOCs，乙苯、二甲苯、苯甲醛和苯乙烯）释放到培养基中。总之，数据表明幼虫卵黄吸收延迟，这可能归因于VOCs的释放，VOCs会诱导氧化应激。鉴于这一发现潜在的生物学和生态学意义，迫切需要进一步研究。
