Yang Dewei, Jin Yidan, He Niqing, Lin Shaojun, Cheng Zhaoping, Huang Fenghuang, Zhang Haifeng, Li Qingshun Q, Yu Wenquan
Institute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China.
Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China.
Plants (Basel). 2025 Aug 18;14(16):2566. doi: 10.3390/plants14162566.
Rice blast is one of the main diseases of rice, causing severe economic losses to agricultural production; thus, the search for blast resistance is a top priority for rice breeding. When challenged by the blast causal fungus the expression level of gene in rice cultivar Nipponbar was found to increase significantly. Such an induction was also found in a different genetic material, cultivar Shufanggaonuo, indicating that plays an important role in blast disease response. However, the function of remains unclear. In this study, wild type ZH11 was selected as the background material to investigate the expression and functions of in rice disease resistance by constructing knockout mutants. The results showed that is mainly expressed in and localized in cell membrane, cytoplasm, and nucleus. The two allelic knockout mutants, and , were more resistant to and bacterial blight pv. (). Further agronomic trait analysis revealed that the and mutants had reduced plant height, smaller grain size, a significant increase in tillering number, but also a significant increase in yield per plant. Our results show that is involved in the immune response of rice by negatively regulating the resistance to rice blast and blight diseases, and in regulating important agronomic traits. This study lays a foundation for revealing the molecular mechanism of in the immune response to rice diseases and provides novel genetic resources for rice breeding.
稻瘟病是水稻的主要病害之一，给农业生产造成严重经济损失；因此，寻找抗稻瘟病能力是水稻育种的首要任务。当受到稻瘟病致病真菌的挑战时，发现水稻品种日本晴中 基因的表达水平显著增加。在另一种遗传材料品种蜀方高糯中也发现了这种诱导现象，表明 在稻瘟病反应中起重要作用。然而， 的功能仍不清楚。在本研究中，选择野生型ZH11作为背景材料，通过构建敲除突变体来研究 在水稻抗病性中的表达和功能。结果表明， 主要在 中表达，定位于细胞膜、细胞质和细胞核。两个等位基因敲除突变体 和 对 和白稻瘟病和白叶枯病 pv. （ ）更具抗性。进一步的农艺性状分析表明， 和 突变体的株高降低，粒型变小，分蘖数显著增加，单株产量也显著增加。我们的结果表明， 通过负向调节对稻瘟病和白叶枯病的抗性参与水稻的免疫反应，并调节重要的农艺性状。本研究为揭示 在水稻病害免疫反应中的分子机制奠定了基础，并为水稻育种提供了新的遗传资源。
