Coronel-Pastor Lizbeth, Villarreal María Luisa, Zamilpa Alejandro, Herrera-Ruiz Maribel, González-Cortazar Manases, Alvarez Laura, Perea-Arango Irene, Moreno-Anzúrez Norma Elizabeth, Rodríguez Monroy Mario, Arellano-García José de Jesús
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca CP 62209, Morelos, Mexico.
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Xochitepec CP 62790, Morelos, Mexico.
Plants (Basel). 2025 Aug 20;14(16):2585. doi: 10.3390/plants14162585.
Cav., commonly known as "cancer herb" in indigenous communities, has long been used for its medicinal properties. The biotechnological production of its bioactive compounds through genetic transformation represents a valuable approach for obtaining pharmacologically relevant substances. The initial focus of this study was to identify compounds previously reported in the species; however, phytochemical analysis by HPLC and NMR led to the isolation and identification of two pentacyclic triterpene esters not previously described in : 3-O-[(E)-feruloyl]-maslinic acid () and 3-O-[(E)-feruloyl]-corosolic acid (), identified as constituents of fraction 33. The LRTC3.1 callus line was obtained from hairy roots generated by infecting leaf explants with strain ATCC15834/pTDT. The crude extract, specific fractions, and the mixture of these compounds demonstrated significant anti-inflammatory and cytotoxic activities. Anti-inflammatory activity was evaluated using the carrageenan-induced mouse paw edema model, where the crude extract achieved 51.02% inhibition of inflammation compared to meloxicam (30.86%). Cytotoxicity was assessed against three human cancer cell lines: breast carcinoma (MCF7), cervical carcinoma (SiHa), and colon carcinoma (HCT-15). Fractions FD (28-29) and 33 exhibited potent cytotoxic effects, with IC values of 0.508 and 1.345 µg/mL against SiHa cells, and 0.053 and 2.693 µg/mL against MCF-7 cells, respectively. These findings suggest that transformed cultures represent a promising source of bioactive compounds for potential therapeutic development.
Cav.在当地社区通常被称为“抗癌草药”，长期以来因其药用特性而被使用。通过基因转化对其生物活性化合物进行生物技术生产是获得药理相关物质的一种有价值的方法。本研究最初的重点是鉴定该物种中先前报道的化合物；然而，通过高效液相色谱法（HPLC）和核磁共振（NMR）进行的植物化学分析导致分离和鉴定出两种先前未在该物种中描述的五环三萜酯：3 - O - [(E) - 阿魏酰基] - 山楂酸（）和3 - O - [(E) - 阿魏酰基] - 科罗索酸（），它们被鉴定为馏分33的成分。LRTC3.1愈伤组织系是通过用ATCC15834/pTDT菌株感染叶片外植体产生的毛状根获得的。粗提物、特定馏分以及这些化合物的混合物表现出显著的抗炎和细胞毒性活性。使用角叉菜胶诱导的小鼠爪肿胀模型评估抗炎活性，与美洛昔康（30.86%）相比，粗提物对炎症的抑制率达到51.02%。针对三种人类癌细胞系评估细胞毒性：乳腺癌（MCF7）、宫颈癌（SiHa）和结肠癌（HCT - 15）。馏分FD（28 - 29）和33表现出强大的细胞毒性作用，对SiHa细胞的IC值分别为0.508和1.345 µg/mL，对MCF - 7细胞的IC值分别为0.053和2.693 µg/mL。这些发现表明，转化后的培养物是潜在治疗开发中生物活性化合物的有前景的来源。
