Sorsa M, Einistö P, Husgafvel-Pursiainen K, Järventaus H, Kivistö H, Peltonen Y, Tuomi T, Valkonen S, Pelkonen O
J Toxicol Environ Health. 1985;16(3-4):523-34. doi: 10.1080/15287398509530760.
Six volunteer female habitual smokers were exposed during a 2-wk experimental period to cigarette smoke, both actively and passively, in an exposure chamber (volume 10 m3, average air exchange rate 6.8 times/h), where the ambient carbon monoxide, particle, and aldehyde concentrations were monitored. Three of the six subjects were smoking at the time, 2 cigarettes (filtered, self-burning low tar brand) per person per hour, 30 cigarettes altogether during each of the 5-h experimental days in the chamber. Samples of blood and urine were taken from each subject after 3 nonsmoking days and after each day of active or passive smoking. Among the parameters tested, blood carboxyhemoglobin, plasma cotinine, and urinary mutagenicity were higher in samples taken after active smoking than after nonsmoking periods. Although the exposure conditions were similar for all subjects, the parameters measured showed quite high interindividual variation. Thioethers and thiocyanates were not significantly elevated in the active smoking samples; neither were there any differences during this short experimental period in the sister chromatid exchange frequencies. The only parameters showing an increasing trend after passive exposure, as compared with nonsmoking samples, were urinary mutagenicity and plasma cotinine, the main metabolite of nicotine.
6名有吸烟习惯的女性志愿者在一个2周的实验期内，在一个暴露舱（容积10立方米，平均换气率6.8次/小时）中主动和被动地接触香烟烟雾，在该暴露舱中监测环境一氧化碳、颗粒物和醛类浓度。6名受试者中有3名在实验期间吸烟，每人每小时吸2支香烟（过滤嘴、自燃低焦油品牌），在暴露舱内进行的5小时实验日中，每天共吸30支香烟。在3个非吸烟日之后以及主动或被动吸烟的每一天之后，从每个受试者采集血液和尿液样本。在测试的参数中，主动吸烟后采集的样本中的血液碳氧血红蛋白、血浆可替宁和尿致突变性高于非吸烟期之后。尽管所有受试者的暴露条件相似，但所测量的参数显示出相当大的个体间差异。硫醚和硫氰酸盐在主动吸烟样本中没有显著升高；在这个短实验期内，姐妹染色单体交换频率也没有差异。与非吸烟样本相比，被动暴露后唯一呈上升趋势的参数是尿致突变性和血浆可替宁，后者是尼古丁的主要代谢产物。
