Ono T, Okada S, Kawakami T, Honjo T, Getz M J
Mech Ageing Dev. 1985 Nov;32(2-3):227-34. doi: 10.1016/0047-6374(85)90082-x.
The possibility of age-associated changes in DNA sequence in terms of amplification and rearrangement was examined in mouse spleen, liver and brain using the method of Southern transfer and filter hybridization. The DNA regions studied were at and around nine cloned sequences, most of which are known to move or amplify in certain situations. No detectable age-associated change, however, was observed in all DNA regions studied. These results suggest that widespread DNA sequence rearrangements or amplifications do not occur during the ageing process in mice.
运用Southern印迹转移和滤膜杂交方法，对小鼠脾脏、肝脏和大脑中DNA序列在扩增和重排方面与年龄相关的变化可能性进行了检测。所研究的DNA区域位于9个克隆序列及其周围，其中大多数已知在某些情况下会移动或扩增。然而，在所研究的所有DNA区域中均未观察到可检测到的与年龄相关的变化。这些结果表明，在小鼠衰老过程中不会发生广泛的DNA序列重排或扩增。
