Chahardehi Farzaneh, Khani Rouhollah, Farrokhi Alireza
Department of Chemistry, Faculty of Science, University of Birjand, Birjand, 97179-414, Iran.
Department of Chemistry, Faculty of Science, University of Birjand, Birjand, 97179-414, Iran.
Food Chem Toxicol. 2025 Nov;205:115715. doi: 10.1016/j.fct.2025.115715. Epub 2025 Aug 28.
Enrofloxacin (ENR), a broad-spectrum fluoroquinolone antibiotic, is widely used in both human and veterinary medicine. However, residual ENR in food and water can pose significant health risks. In this study, a novel bimetallic zeolitic imidazolate framework (ZIF-67@ZIF-8) a type of metal-organic framework (MOF), was synthesized at room temperature, and employed as an effective sorbent in a dispersive micro-solid phase extraction (Dμ-SPE) procedure for the rapid and sensitive detection of ENR. The synthesized metal-organic framework-on-metal-organic framework (MOF-on-MOF) material was characterized using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Brunauer-Emmett-Teller (BET) analysis. Key experimental parameters, including pH, sorbent amount, adsorption time, and salt concentration, were optimized using a central composite design (CCD). Under optimal conditions, the method showed a linear dynamic range (LDR) of 5-600 ng mL, a limit of detection (LOD) of 1.43 ng mL, a limit of quantification (LOQ) of 4.71 ng mL, and an intra-day relative standard deviation (RSD) of 3.55 % (n = 3). Satisfactory recoveries ranging from 89.37 % to 101.10 % with RSD values below 6.9 % were achieved for ENR determination in food and water samples.
恩诺沙星（ENR）是一种广谱氟喹诺酮类抗生素，广泛应用于人类医学和兽医学。然而，食品和水中残留的恩诺沙星会带来重大健康风险。在本研究中，一种新型双金属沸石咪唑酯骨架材料（ZIF-67@ZIF-8），即一种金属有机骨架材料（MOF），在室温下合成，并用作分散微固相萃取（Dμ-SPE）程序中的有效吸附剂，用于快速灵敏地检测恩诺沙星。采用傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、能量色散X射线光谱（EDS）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和布鲁诺尔-埃米特-泰勒（BET）分析对合成的金属有机骨架-金属有机骨架（MOF-on-MOF）材料进行了表征。使用中心复合设计（CCD）对包括pH值、吸附剂用量、吸附时间和盐浓度在内的关键实验参数进行了优化。在最佳条件下，该方法的线性动态范围（LDR）为5-600 ng mL，检测限（LOD）为1.43 ng mL，定量限（LOQ）为4.71 ng mL，日内相对标准偏差（RSD）为3.55%（n = 3）。在食品和水样中测定恩诺沙星时，回收率令人满意，范围为89.37%至101.10%，RSD值低于6.9%。
