Dąbek Józefa, Głogowska-Ligus Joanna, Piechota Mieczysław
Department of Cardiology, Faculty of Health Sciences in Katowice, Medical University of Silesia, Katowice, Poland.
Department of Epidemiology, Faculty of Public Health in Bytom, Medical University of Silesia, Katowice, Poland.
J Cell Mol Med. 2025 Sep;29(17):e70727. doi: 10.1111/jcmm.70727.
The aim of the study was to analyse and compare the transcriptional activity of the TGF-β1 gene and its receptors in patients with heart failure based on coronary artery disease and patients with coronary artery disease without heart failure, taking into account the presence of individual risk factors, the stage of heart failure and the number of diseased coronary arteries. The study included 105 patients with advanced heart failure (NYHA III-IV) and 52 patients with coronary artery disease without heart failure. The study was performed using the QRT-PCR technique. The transcriptional activity of the TGF-β1 gene and its type III receptor was significantly lower in patients with advanced heart failure. In this group of patients with risk factors such as previous myocardial infarction, family history, overweight/obesity and arterial hypertension, a statistically significant reduction in TGF-β1 gene expression was demonstrated. In patients with heart failure, changes in the transcriptional activity of all receptor's genes were found when these patients were burdened with risk factors. The reduced transcriptional activity of TGF-β1 and its type III receptor demonstrated in patients with heart failure may be a useful marker in clinical practice for assessing the progression of coronary artery disease towards the development of heart failure, as well as its decompensation and compensation.
本研究的目的是分析和比较基于冠状动脉疾病的心力衰竭患者与无心力衰竭的冠状动脉疾病患者中转化生长因子-β1（TGF-β1）基因及其受体的转录活性，同时考虑个体危险因素的存在、心力衰竭的阶段以及病变冠状动脉的数量。该研究纳入了105例晚期心力衰竭（纽约心脏协会III-IV级）患者和52例无心力衰竭的冠状动脉疾病患者。研究采用实时定量聚合酶链反应（QRT-PCR）技术进行。晚期心力衰竭患者中TGF-β1基因及其III型受体的转录活性显著降低。在这组具有既往心肌梗死、家族史、超重/肥胖和动脉高血压等危险因素的患者中，TGF-β1基因表达有统计学意义的降低。在心力衰竭患者中，当这些患者存在危险因素时，发现所有受体基因的转录活性均发生变化。心力衰竭患者中TGF-β1及其III型受体转录活性降低，可能是临床实践中评估冠状动脉疾病向心力衰竭发展进程及其失代偿和代偿情况的有用标志物。
