Lee Yeong-Geun, Kwon Jeong Eun, Choi Won-Sil, Baek Nam-In, Kang Se Chan
Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology Kyung Hee University Yongin Korea.
National Instrumentation Center for Environmental Management Seoul National University Seoul Korea.
Plant Direct. 2024 Sep 19;8(9):e616. doi: 10.1002/pld3.616. eCollection 2024 Sep.
(Oleaceae), endemic to the Korean Peninsula and the sole member of its genus and species, possesses high scarcity value, escalating its importance under the Nagoya Protocol. Despite its significance, their metabolites and activities of flowers remain unexplored. This study employs an integrated metabolomic approach utilizing NMR, LC/MS, GC/MS, and FTIR techniques to comprehensively analyze the metabolite profile of flowers. By combining these methods, we identified 35 metabolites, 43 secondary metabolites, and 108 hydrophobic primary metabolites. Notably, distinct concentration patterns of these compounds were observed across five variants, classified based on morphological characteristics. Correlation analyses of primary and secondary metabolites unveiled varietal metabolic flux, providing insights into flower metabolism. Additionally, the reconstruction of metabolic pathways based on dissimilarities in morphological traits elucidates variant-specific metabolic signatures. These findings not only enhance our understanding of chemical differences between varieties but also underscore the importance of considering varietal differences in future research and conservation efforts.
（木犀科），原产于朝鲜半岛，是其属和种的唯一成员，具有很高的稀缺价值，根据《名古屋议定书》其重要性不断提升。尽管其具有重要意义，但其花朵的代谢产物和活性仍未得到探索。本研究采用综合代谢组学方法，利用核磁共振（NMR）、液相色谱/质谱（LC/MS）、气相色谱/质谱（GC/MS）和傅里叶变换红外光谱（FTIR）技术全面分析花朵的代谢产物谱。通过结合这些方法，我们鉴定出35种代谢产物、43种次生代谢产物和108种疏水性初级代谢产物。值得注意的是，在根据形态特征分类的五个变种中观察到了这些化合物的不同浓度模式。初级和次生代谢产物的相关性分析揭示了品种代谢通量，为花朵代谢提供了见解。此外，基于形态特征差异重建代谢途径阐明了变种特异性代谢特征。这些发现不仅增进了我们对品种间化学差异的理解，也强调了在未来研究和保护工作中考虑品种差异的重要性。
