Basgul Cemile, DeSantis Paul, Derr Tabitha, Hickok Noreen J, Bock Ryan M, Kurtz Steven M
Implant Research Core, School of Biomedical Science, Engineering, and Health Systems, Drexel University, Philadelphia, United States of America.
Department of Orthopedics, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, United States of America.
Int J Bioprint. 2024;10(2). doi: 10.36922/ijb.2124. Epub 2024 Feb 26.
In this study, our goal was to assess the suitability of a polyether-ether-ketone (PEEK) and silicon nitride (SiN) polymer composite for antimicrobial three-dimensional (3D)-printed cervical cages. Generic cage designs (PEEK and 15 vol.% SiN-PEEK) were 3D-printed, including solid and porous cage designs. Cages were tested in static compression, compression shear, and torsion per ASTM F2077. For antibacterial testing, virgin and composite filament samples were inoculated with and . cell testing was conducted using MC3T3-E1 mouse preosteoblasts, where cell proliferation, cumulative mineralization, and osteogenic activity were measured. The 3D-printed PEEK and SiN-PEEK cages exhibited adequate mechanical strength for all designs, exceeding 14.7 kN in compression and 6.9 kN in compression shear. SiN-PEEK exhibited significantly lower bacterial adhesion levels, with a 93.9% reduction (1.21 log), and enhanced cell proliferation when compared to PEEK. SiN-PEEK would allow for custom fabrication of 3D-printed spinal implants that reduce the risk of infection compared to unfilled PEEK or metallic alloys.
在本研究中，我们的目标是评估聚醚醚酮（PEEK）和氮化硅（SiN）聚合物复合材料用于抗菌三维（3D）打印颈椎椎间融合器的适用性。对通用椎间融合器设计（PEEK和15体积% SiN-PEEK）进行了3D打印，包括实心和多孔椎间融合器设计。根据ASTM F2077标准，对椎间融合器进行了静态压缩、压缩剪切和扭转测试。对于抗菌测试，将原始和复合长丝样品接种 和 。使用MC3T3-E1小鼠前成骨细胞进行细胞测试，测量细胞增殖、累积矿化和成骨活性。所有设计的3D打印PEEK和SiN-PEEK椎间融合器均表现出足够的机械强度，压缩强度超过14.7 kN，压缩剪切强度超过6.9 kN。与PEEK相比，SiN-PEEK的细菌粘附水平显著降低，降低了93.9%（1.21对数），并且细胞增殖增强。与未填充的PEEK或金属合金相比，SiN-PEEK将允许定制制造3D打印脊柱植入物，降低感染风险。
