Mavlonazarova Sylhiya, Acosta Kenneth, Abzalimov Rinat, Satorov Saidbeg, Dushenkov Vyacheslav
Medical - Social Institute of Tajikistan, Dushanbe, Republic of Tajikistan.
Department of Plant Biology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA.
bioRxiv. 2025 Aug 27:2025.08.24.671964. doi: 10.1101/2025.08.24.671964.
Korovin, an endemic Tajikistani plant with purported medicinal properties, remains understudied. This study employs untargeted metabolomics to characterize the metabolite profiles of ethanol extracts and juices from roots and seeds. In total, 540 distinct metabolites are putatively identified, 419 of which are previously unreported in the genus, representing a substantial expansion of its known chemical diversity. The most abundant metabolites are terpenoids, amino acid derivatives, and alkaloids. A particularly abundant group of daucane sesquiterpenoids, sharing a common (6-methyl-azulen-4-yl)cyclohexanecarboxylate substructure, is identified, including known metabolites such as ferutidin and ferutinin. Comparative analysis reveals organ-specific metabolic specialization: roots are enriched in terpenoids, whereas seeds exhibit higher concentrations of alkaloids and amino acids. Additionally, processing methods influence metabolite composition, with ethanol extracts being rich in terpenoids and amino acids, and juices displaying a greater diversity of phenylpropanoid-derived compounds. These findings expand the phytochemical richness of and suggest its potential as a valuable source of bioactive compounds for pharmacological exploration.
科罗温是塔吉克斯坦的一种本土植物，据称具有药用特性，但仍未得到充分研究。本研究采用非靶向代谢组学方法来表征根和种子的乙醇提取物及汁液的代谢物谱。总共推定鉴定出540种不同的代谢物，其中419种在该属中此前未被报道，这极大地扩展了其已知的化学多样性。含量最丰富的代谢物是萜类化合物、氨基酸衍生物和生物碱。鉴定出了一组特别丰富的胡萝卜烷倍半萜类化合物，它们具有共同的（6-甲基-薁-4-基）环己烷羧酸酯亚结构，包括诸如阿魏苷和阿魏宁等已知代谢物。比较分析揭示了器官特异性的代谢特化：根中富含萜类化合物，而种子中生物碱和氨基酸的浓度更高。此外，加工方法会影响代谢物组成，乙醇提取物富含萜类化合物和氨基酸，而汁液中苯丙烷类衍生化合物的多样性更高。这些发现扩展了该植物的植物化学丰富度，并表明其作为药理探索中有价值的生物活性化合物来源的潜力。
