Savolainen H, Pfäffli P, Elovaara E
Arch Toxicol. 1985 Sep;57(4):285-8. doi: 10.1007/BF00324794.
Male Wistar rats exposed to 50, 100 or 300 ppm methyl tertiary-butyl ether vapour for 2-15 weeks, 6 h daily, 5 days a week, showed a dose-dependent blood ether concentration after 2 weeks' exposure. Blood concentrations of tertiary-butanol, were also dose dependent indicating metabolic breakdown of the ether in vivo. The blood ether concentrations decreased after 6 weeks of exposure at the 50 ppm dose level and remained unaffected at higher doses while tertiary-butanol concentrations increased after 6 weeks with all doses, and began to decrease thereafter. Exposure caused a transient increase in UDP-glucuronosyltransferase activities in liver and kidney microsomes, almost no effects on hepatic cytochrome P-450 concentrations and a minor induction of kidney microsomal cytochrome P-450 content. Exposure produced almost no effect on brain succinate dehydrogenase, creatine kinase or acetylcholinesterase activities, while early inhibition of muscle creatine kinase activity was noted, accompanied by increased activity at the end of exposure.
雄性Wistar大鼠每天暴露于50、100或300 ppm甲基叔丁基醚蒸气中6小时，每周5天，持续2至15周。暴露2周后，血液中的醚浓度呈剂量依赖性。叔丁醇的血液浓度也呈剂量依赖性，表明醚在体内发生了代谢分解。在50 ppm剂量水平下暴露6周后，血液中的醚浓度下降，而在较高剂量下则不受影响；而所有剂量在暴露6周后叔丁醇浓度均升高，此后开始下降。暴露导致肝脏和肾脏微粒体中的UDP-葡萄糖醛酸转移酶活性短暂增加，对肝细胞色素P-450浓度几乎没有影响，对肾脏微粒体细胞色素P-450含量有轻微诱导作用。暴露对脑琥珀酸脱氢酶、肌酸激酶或乙酰胆碱酯酶活性几乎没有影响，而早期观察到肌肉肌酸激酶活性受到抑制，暴露结束时活性增加。
