Zhu Qinchao, Zhou Xuhao, Wang Zhidan, Ren Daxi, Ren Tanchen
Department of Cardiology The Second Affiliated Hospital School of Medicine Institute of Dairy Science College of Animal Sciences Zhejiang University Hangzhou 310009 China.
State Key Laboratory of Transvascular Implantation Devices Heart Regeneration and Repair Key Laboratory of Zhejiang Province Hangzhou 310009 China.
Small Sci. 2025 Jun 23;5(9):2500026. doi: 10.1002/smsc.202500026. eCollection 2025 Sep.
The emergence of drug-resistant bacteria due to excessive antibiotic use has drawn increasing attention to inorganic nanoparticles for their broad-spectrum antibacterial properties. Here, a "green" strategy for the simultaneous in situ synthesis of silver nanoparticles (AgNPs) during the photocrosslinking process of casein hydrogels is described. The in situ photoactivated biomineralization of AgNPs provides noticeable stability and antibacterial activity, with high photothermal effect during a sequential near-infrared laser activation. The milk-derived casein is screened out due to its great biomineralization capability and wound healing activity. Casein-AgNP hydrogel dressing shows low swelling, good mechanical properties, and nice biocompatibility. In animal experiments, casein-AgNP hydrogel accelerates wound repair and tissue regeneration after bacterial infection by regulating immune response. Our work shows that sequential photoactivation served as a promising strategy for antiinfectious wound treatment, and casein hydrogel stood as a potential candidate for in situ biomineralization.
由于抗生素的过度使用导致耐药细菌的出现，无机纳米颗粒因其广谱抗菌特性而受到越来越多的关注。在此，描述了一种在酪蛋白水凝胶光交联过程中同时原位合成银纳米颗粒（AgNPs）的“绿色”策略。AgNPs的原位光活化生物矿化提供了显著的稳定性和抗菌活性，在连续近红外激光激活过程中具有高光热效应。由于其强大的生物矿化能力和伤口愈合活性，筛选出了源自牛奶的酪蛋白。酪蛋白-AgNP水凝胶敷料显示出低肿胀、良好的机械性能和良好的生物相容性。在动物实验中，酪蛋白-AgNP水凝胶通过调节免疫反应加速细菌感染后的伤口修复和组织再生。我们的工作表明，连续光活化是一种有前景的抗感染伤口治疗策略，酪蛋白水凝胶是原位生物矿化的潜在候选材料。
