大麦醇溶蛋白的13C核磁共振研究
13C-n.m.r. study of C hordein.
作者信息
Tatham A S, Shewry P R, Belton P S
出版信息
Biochem J. 1985 Dec 1;232(2):617-20. doi: 10.1042/bj2320617.
Abstract
The structure of C hordein was studied by a combination of solution and solid-state 13C-n.m.r. spectroscopy. The repetitive primary structure results in simple solution-state n.m.r. spectra, which allow assignment of the majority of the resonances to five major residues. The major resonances, with the exception of the aromatic signals, are also present in the solid-state spectra. The proline residues are in the trans configuration, consistent with an earlier study suggesting a beta-turn-rich structure.
摘要
通过溶液态和固态(^{13}C)核磁共振光谱相结合的方法研究了大麦醇溶蛋白的结构。其重复的一级结构导致溶液态核磁共振光谱简单，这使得大部分共振信号可归属于五个主要残基。除芳香族信号外，主要共振信号也出现在固态光谱中。脯氨酸残基处于反式构型，这与早期一项表明富含β-转角结构的研究一致。
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