一种富含谷氨酰胺和脯氨酸的谷物种子蛋白——大麦醇溶蛋白的构象研究。
A conformational study of a glutamine- and proline-rich cereal seed protein, C hordein.
作者信息
Tatham A S, Drake A F, Shewry P R
出版信息
Biochem J. 1985 Mar 1;226(2):557-62. doi: 10.1042/bj2260557.
Abstract
A combination of c.d. spectroscopy and computer prediction is used to show that C hordein has an unusual secondary structure with an absence of alpha-helix and beta-sheet, but the presence of regularly repeated beta-turns. This is associated with a repetitive primary structure based mainly on blocks of eight residues. Similar spectral changes occurred when the protein was heated from 6 to 86 degrees C in aq. 70% (v/v) ethanol or dissolved in increasing concentrations (50-100%, v/v) of trifluoroethanol in water. The studies indicated that the conformation is stabilized by strong hydrophobic interactions and by extensive hydrogen-bonding.
摘要
圆二色光谱法和计算机预测相结合的方法被用于表明大麦醇溶蛋白具有不寻常的二级结构，其缺乏α-螺旋和β-折叠，但存在规则重复的β-转角。这与主要基于八个残基组成的重复一级结构相关。当蛋白质在70%（v/v）乙醇水溶液中从6℃加热到86℃，或溶解于水相中浓度不断增加（50 - 100%，v/v）的三氟乙醇中时，会出现类似的光谱变化。研究表明，该构象通过强烈的疏水相互作用和广泛的氢键作用得以稳定。
相似文献
1
A conformational study of a glutamine- and proline-rich cereal seed protein, C hordein.一种富含谷氨酰胺和脯氨酸的谷物种子蛋白——大麦醇溶蛋白的构象研究。
Biochem J. 1985 Mar 1;226(2):557-62. doi: 10.1042/bj2260557.
2
Conformational studies of a synthetic peptide corresponding to the repeat motif of C hordein.对应于大麦醇溶蛋白重复基序的合成肽的构象研究。
Biochem J. 1989 Apr 15;259(2):471-6. doi: 10.1042/bj2590471.
3
Identification of a novel beta-turn-rich repeat motif in the D hordeins of barley.在大麦D hordeins中鉴定出一种新的富含β-转角的重复基序。
Biochim Biophys Acta. 1992 Jul 31;1122(2):118-22. doi: 10.1016/0167-4838(92)90313-3.
4
Antioxidant capacities of fractionated barley hordein hydrolysates in relation to peptide structures.大麦醇溶蛋白分步水解产物的抗氧化能力与其肽结构的关系。
Mol Nutr Food Res. 2013 Mar;57(3):493-503. doi: 10.1002/mnfr.201200252. Epub 2013 Jan 14.
5
Temperature-dependent binding of monoclonal antibodies to C hordein.
Biochim Biophys Acta. 2002 Jan 31;1594(1):17-26. doi: 10.1016/s0167-4838(01)00269-2.
6
Secondary structure prediction of seed storage proteins.种子贮藏蛋白的二级结构预测
Int J Pept Protein Res. 1983 Oct;22(4):456-63. doi: 10.1111/j.1399-3011.1983.tb02115.x.
7
Targeted modification of storage protein content resulting in improved amino acid composition of barley grain.对贮藏蛋白含量进行靶向修饰，从而改善大麦籽粒的氨基酸组成。
Transgenic Res. 2016 Feb;25(1):19-31. doi: 10.1007/s11248-015-9911-7. Epub 2015 Oct 27.
8
Conformation of the RNA polymerase II C-terminal domain: circular dichroism of long and short fragments.RNA聚合酶II羧基末端结构域的构象：长短片段的圆二色性
J Mol Biol. 2000 Mar 17;297(1):119-33. doi: 10.1006/jmbi.2000.3545.
9
Short tandem repeats shared by B- and C-hordein cDNAs suggest a common evolutionary origin for two groups of cereal storage protein genes.B-和C-大麦醇溶蛋白cDNA共有的短串联重复序列表明两组谷物贮藏蛋白基因有共同的进化起源。
EMBO J. 1985 Jan;4(1):9-15. doi: 10.1002/j.1460-2075.1985.tb02310.x.
10
13C-n.m.r. study of C hordein.大麦醇溶蛋白的13C核磁共振研究
Biochem J. 1985 Dec 1;232(2):617-20. doi: 10.1042/bj2320617.
引用本文的文献
1
Identification of methionine -rich insoluble proteins in the shell of the pearl oyster, Pinctada fucata.鉴定珍珠贝（Pinctada fucata）壳中富含蛋氨酸的不溶性蛋白质。
Sci Rep. 2020 Oct 27;10(1):18335. doi: 10.1038/s41598-020-75444-4.
2
Gliadins from wheat grain: an overview, from primary structure to nanostructures of aggregates.小麦籽粒中的醇溶蛋白：概述，从一级结构到聚集体的纳米结构
Biophys Rev. 2018 Apr;10(2):435-443. doi: 10.1007/s12551-017-0367-2. Epub 2017 Dec 4.
3
Properties of Gluten Intolerance: Gluten Structure, Evolution, Pathogenicity and Detoxification Capabilities.不耐受麸质的特性：麸质结构、进化、致病性和解毒能力。
Nutrients. 2016 Oct 18;8(10):644. doi: 10.3390/nu8100644.
4
Fourier transform IR spectroscopic study of hydration-induced structure changes in the solid state of omega-gliadins.傅里叶变换红外光谱法研究ω-醇溶蛋白固态水合诱导的结构变化
Biochem J. 1996 Nov 1;319 ( Pt 3)(Pt 3):741-7. doi: 10.1042/bj3190741.
5
The structure and function of proline-rich regions in proteins.蛋白质中富含脯氨酸区域的结构与功能。
Biochem J. 1994 Jan 15;297 ( Pt 2)(Pt 2):249-60. doi: 10.1042/bj2970249.
6
A new Escherichia coli cell division gene, ftsK.一个新的大肠杆菌细胞分裂基因，ftsK。
J Bacteriol. 1995 Nov;177(21):6211-22. doi: 10.1128/jb.177.21.6211-6222.1995.
7
13C-n.m.r. study of C hordein.大麦醇溶蛋白的13C核磁共振研究
Biochem J. 1985 Dec 1;232(2):617-20. doi: 10.1042/bj2320617.
8
The structure of a high-Mr subunit of durum-wheat (Triticum durum) gluten.硬粒小麦（Triticum durum）面筋的高分子量亚基结构。
Biochem J. 1987 Oct 1;247(1):215-21. doi: 10.1042/bj2470215.
9
Conformational studies of a synthetic peptide corresponding to the repeat motif of C hordein.对应于大麦醇溶蛋白重复基序的合成肽的构象研究。
Biochem J. 1989 Apr 15;259(2):471-6. doi: 10.1042/bj2590471.
10
The prolamin storage proteins of cereal seeds: structure and evolution.谷物种子的醇溶蛋白贮藏蛋白：结构与进化
Biochem J. 1990 Apr 1;267(1):1-12. doi: 10.1042/bj2670001.
本文引用的文献
1
Conformations of synthetic alamethicin fragments. Evidence for 310 helical folding from 270-MHz hydrogen-1 nuclear magnetic resonance and circular dichroism studies.合成短杆菌肽片段的构象。来自270兆赫氢-1核磁共振和圆二色性研究的关于310螺旋折叠的证据。
Biochemistry. 1981 May 12;20(10):2828-35. doi: 10.1021/bi00513a019.
2
Characterization of soluble peptides of elastin by physical techniques.用物理技术对弹性蛋白可溶性肽进行表征。
Methods Enzymol. 1982;82 Pt A:673-716. doi: 10.1016/0076-6879(82)82096-x.
3
Reverse turns in peptides and proteins.肽和蛋白质中的反向转角
CRC Crit Rev Biochem. 1980;8(4):315-99. doi: 10.3109/10409238009105470.
4
Conformation of model, alanine and proline containing tetrapeptides in water. Comparison of 13C-n.m.r. and CD results.
Int J Pept Protein Res. 1983 Mar;21(3):301-6. doi: 10.1111/j.1399-3011.1983.tb03108.x.
5
Conformational study of the salivary proline-rich polypeptides.
Int J Pept Protein Res. 1983 Mar;21(3):281-7. doi: 10.1111/j.1399-3011.1983.tb03105.x.
6
Conformational study of the basic proline-rich polypeptides from human parotid saliva.人腮腺唾液中富含脯氨酸的碱性多肽的构象研究。
Int J Pept Protein Res. 1984 Feb;23(2):158-65. doi: 10.1111/j.1399-3011.1984.tb02706.x.
7
Molecular analysis of a mutation conferring the high-lysine phenotype on the grain of barley (Hordeum vulgare).赋予大麦（Hordeum vulgare）籽粒高赖氨酸表型的突变的分子分析。
Cell. 1983 Aug;34(1):161-7. doi: 10.1016/0092-8674(83)90146-0.
8
Stereochemical criteria for polypeptides and proteins. V. Conformation of a system of three linked peptide units.多肽和蛋白质的立体化学标准。V. 三个相连肽单元系统的构象
Biopolymers. 1968 Oct;6(10):1425-36. doi: 10.1002/bip.1968.360061006.
9
Computed circular dichroism spectra for the evaluation of protein conformation.用于评估蛋白质构象的计算圆二色光谱。
Biochemistry. 1969 Oct;8(10):4108-16. doi: 10.1021/bi00838a031.
10
Protein denaturation. C. Theoretical models for the mechanism of denaturation.蛋白质变性。C. 变性机制的理论模型。
Adv Protein Chem. 1970;24:1-95.
Zotero 插件