Choudhury Anurupa, Mahanta Anindita
Forensic Medicine, Dhubri Medical College, Dhubri, IND.
Physiology, All India Institute of Medical Sciences, Guwahati, Guwahati, IND.
Cureus. 2025 Aug 11;17(8):e89773. doi: 10.7759/cureus.89773. eCollection 2025 Aug.
There are several methods to estimate time since death, but no method, used singly, is totally reliable. After death, several morphological changes occur in all the cellular components of blood, including the white blood cells. In this study, blood samples were collected from 20 non-refrigerated cadavers, with known time and cause of death and without any hematological, oncological, or infectious disease, brought to the mortuary of a tertiary care hospital. One blood film was prepared and stained immediately, and subsequent blood films were made and stained at regular intervals up to 120 hours. It was observed that lymphocytes were most resistant and neutrophils were most vulnerable to autolytic changes following death. Observing the sequential changes occurring in the leucocytes in the postmortem period may serve as an additional parameter in determining the time since death.
有几种方法可用于估计死亡时间，但没有一种单独使用的方法是完全可靠的。死后，血液的所有细胞成分（包括白细胞）都会发生一些形态学变化。在本研究中，从20具未冷藏的尸体采集血样，这些尸体的死亡时间和死因已知，且无任何血液学、肿瘤学或传染病，尸体被送至一家三级护理医院的停尸房。立即制备并染色一张血涂片，随后每隔一定时间制备并染色血涂片，直至120小时。观察到淋巴细胞最具抗性，而中性粒细胞在死后最易发生自溶变化。观察死后白细胞中发生的连续变化可作为确定死亡时间的一个额外参数。
