Laiho K, Penttilä A
Forensic Sci Int. 1981 Mar-Apr;17(2):109-20. doi: 10.1016/0379-0738(81)90003-7.
The viability of white blood cells, spermatozoa of the epididymis, cells from minced spleen, lymph node and lung, and cells aspirated from the bone marrow of the sternum was studied using the vital dye exclusion test. The material comprised 123 medicolegal autopsy cadavers which had been stored in a mortuary cold room at +4 degrees C up to 10 days after death. Cells which excluded trypan blue were found in various specimens from all cadavers. However, there was marked individual variation in the results. The loss of viability of the white blood cells showed a moderate correlation (r = -0.78) with the increase in the post-mortem (p.m.) time, whereas the results for other tissues were not so significant. The K+ and Mg2+ and haemoglobin content and the osmotic resistance of the red cells correlated poorly with the p.m. time. The present results suggest that despite the general assumption that autolytic changes proceed rapidly at the cellular level, individual cells and especially blood cells may remain viable for a long time in cadavers kept at +4 degrees C.
使用活染排除试验研究了白细胞、附睾精子、脾脏、淋巴结和肺脏切碎组织中的细胞以及胸骨骨髓吸出细胞的活力。材料包括123例法医解剖尸体，这些尸体在停尸房冷藏室中于死后在4℃保存长达10天。在所有尸体的各种标本中均发现了排斥台盼蓝的细胞。然而，结果存在明显的个体差异。白细胞活力丧失与死后（p.m.）时间的增加呈中度相关（r = -0.78），而其他组织的结果则不那么显著。红细胞的K +、Mg2 +、血红蛋白含量和渗透压抵抗力与死后时间的相关性较差。目前的结果表明，尽管一般认为自溶变化在细胞水平上迅速发生，但在4℃保存的尸体中，单个细胞尤其是血细胞可能长时间保持活力。
