Shinozuka K, Maeda T, Hayashi E
J Pharmacobiodyn. 1985 Oct;8(10):877-84. doi: 10.1248/bpb1978.8.877.
The possibilities of participation of adenosine in the peristaltic reflex of guinea pig ileum was studied pharmacologically and histologically. Adenosine apparently depressed the peristaltic activity induced by elevation of intraluminal pressure from zero to 1-8 cm H2O, and the extent of the depression somewhat decreased in proportion to the elevation in intraluminal pressure. Also, dipyridamole depressed the peristaltic activity by itself; however, the extent of the depression significantly increased in proportion to the elevation in intraluminal pressure. On the other hand, in the presence of dipyridamole, the elevation of the intraluminal pressure from zero to 1-8 cm H2O elicited an 3H-output increase from 3H-adenosine preloaded guinea pig isolated ileum, with the effect being more pronounced as the pressure was increased. In contrast, the peristaltic activity was more pronounced at lower pressurization. Atropine greatly depressed the peristaltic response but did not affect 3H-output induced by 4 cm H2O pressurization. Tetrodotoxin depressed both markedly. Fluorescence histochemical localization of quinacrine, which binds to adenosin triphosphate (ATP), revealed dense nerve cell bodies and fine interconnecting strands in the ileal myenteric plexus of Auerbach. Also, in microradioautographs of ileal longitudinal muscle incubated with 3H-adenosine, the concentration of developed silver grains was localized in the ganglion cells and in the musculature as varicose fibre. From these results, evidence is provided that the peristalsis of guinea pig ileum may be physiologically modulated by endogenous adenosine, which may be released from neuronal elements of the myenteric plexus in response to the applied intraluminal pressure.
采用药理学和组织学方法研究了腺苷参与豚鼠回肠蠕动反射的可能性。腺苷明显抑制由腔内压力从零升高至1 - 8 cm H₂O所诱导的蠕动活动，且抑制程度随腔内压力升高而略有降低。此外，双嘧达莫自身也能抑制蠕动活动；然而，其抑制程度随腔内压力升高而显著增加。另一方面，在双嘧达莫存在的情况下，腔内压力从零升高至1 - 8 cm H₂O会使预先加载³H - 腺苷的豚鼠离体回肠的³H释放量增加，压力越高，效果越明显。相反，在较低压力下蠕动活动更明显。阿托品能极大地抑制蠕动反应，但不影响4 cm H₂O压力诱导的³H释放。河豚毒素对两者均有显著抑制作用。与三磷酸腺苷（ATP）结合的奎纳克林的荧光组织化学定位显示，在奥尔巴赫回肠肌间神经丛中有密集的神经细胞体和细小的相互连接的纤维束。此外，在用³H - 腺苷孵育的回肠纵行肌的显微放射自显影片中，显影银粒的浓度定位于神经节细胞和呈曲张纤维状的肌肉组织中。从这些结果可以看出，有证据表明豚鼠回肠的蠕动可能受到内源性腺苷的生理调节，内源性腺苷可能是肌间神经丛的神经元元件响应所施加的腔内压力而释放的。
