Inagaki N, Tsuruoka N, Goto S, Matsuyama T, Daikoku M, Nagai H, Koda A
J Pharmacobiodyn. 1985 Nov;8(11):958-63. doi: 10.1248/bpb1978.8.958.
Immunoglobulin E (IgE) antibody production against Dermatophagoides farinae extract (mite antigen) was studied in female BALB/c mice. When mice were immunized with mite antigen and aluminium hydroxide gel (alum) intraperitoneally at intervals of 30 d, good primary, secondary and tertiary IgE antibody responses were observed. In the absence of adjuvant, a subcutaneous injection of mite antigen failed to induce the primary IgE antibody response. However, good IgE antibody responses were observed after the secondary immunization given 30 d after the primary immunization. Furthermore, 5 weekly injections of mite antigen alone also induced the IgE antibody production. Intranasal administrations of mite antigen alone also induced the IgE antibody production in mice. Two exposures to mite antigen, intranasally, were sufficient for eliciting low IgE antibody production. The response was significantly potentiated by the administration of islet-activating protein obtained from culture fluids of Bordetella pertussis. These results indicate that the intranasal administration of mite antigen is very effective for eliciting the IgE antibody production.
在雌性BALB/c小鼠中研究了针对粉尘螨提取物（螨抗原）的免疫球蛋白E（IgE）抗体产生情况。当小鼠每隔30天腹腔注射螨抗原和氢氧化铝凝胶（明矾）时，观察到了良好的初次、二次和三次IgE抗体反应。在没有佐剂的情况下，皮下注射螨抗原未能诱导初次IgE抗体反应。然而，在初次免疫30天后进行二次免疫后，观察到了良好的IgE抗体反应。此外，每周5次单独注射螨抗原也诱导了IgE抗体产生。单独经鼻给予螨抗原也诱导了小鼠体内的IgE抗体产生。经鼻两次接触螨抗原足以引发低水平的IgE抗体产生。百日咳博德特氏菌培养液中获得的胰岛激活蛋白的给药显著增强了该反应。这些结果表明，经鼻给予螨抗原对于引发IgE抗体产生非常有效。
