Kudo K, Okudaira H, Miyamoto T, Nakagawa T, Horiuchi Y
J Allergy Clin Immunol. 1978 Jan;61(1):1-9. doi: 10.1016/0091-6749(78)90466-9.
The antibody response to mite antigen in several mouse strains was studied. BALB/c, CBA, C3H/He, and C57B1 strains showed good responses against mite antigen. The AKR strain, on the other hand, showed a relatively poor response. When BALB/c mice were immunized with DNP-mite conjugate in aluminum hydroxide gel (alum), anti-DNP IgE antibody and IgG1 antibody were induced. When these mice were boosted with mite antigen alone in alum, both anti-mite IgE antibody and IgG1 antibody were induced, although these antibodies were not observed after the first immunization. Both IgE antibody responses were high and persistent. Mite antigen was denatured by alkylation and reduction in the presence of 8 M urea. Native antigen remaining in the chemically denatured antigen was removed by an immunoadsorbent method. The modified mite antigen could stimulate the carrier-specific helper cells, at least when it was injected with alum. It was also found that repeated injections of the modified mite antigen resulted in the suppression of an established IgE antibody response against mite antigen. These findings suggest possible clinical application for hyposensitization therapy.
研究了几种小鼠品系对螨抗原的抗体反应。BALB/c、CBA、C3H/He和C57B1品系对螨抗原表现出良好的反应。另一方面，AKR品系的反应相对较差。当用氢氧化铝凝胶（明矾）中的DNP-螨结合物免疫BALB/c小鼠时，可诱导产生抗DNP IgE抗体和IgG1抗体。当用明矾中的单独螨抗原对这些小鼠进行加强免疫时，可诱导产生抗螨IgE抗体和IgG1抗体，尽管在首次免疫后未观察到这些抗体。两种IgE抗体反应都很高且持续存在。螨抗原在8M尿素存在下通过烷基化和还原进行变性。通过免疫吸附法去除化学变性抗原中残留的天然抗原。修饰后的螨抗原至少在与明矾一起注射时能刺激载体特异性辅助细胞。还发现重复注射修饰后的螨抗原会抑制已建立的针对螨抗原的IgE抗体反应。这些发现提示了减敏疗法可能的临床应用。
