El-Sayed Osama, Abd-Elhalim Basma T, Mosa Mohamed A, Hassan Enas A
Plant Pathology Research Institute, Agricultural Research Center, Giza, 12619, Egypt.
Department of Agriculture Microbiology, Faculty of Agriculture, Ain Shams University, Hadayek Shubra El-Khaimah, Cairo, 11241, Egypt.
Sci Rep. 2025 Sep 12;15(1):32504. doi: 10.1038/s41598-025-17705-8.
One area of science that is experiencing rapid growth is nanotechnology. The goal of nanotechnology research is to develop novel, economical, safe, and effective methods for creating nanoparticles. This study presents a green, cost-effective, and environmentally friendly method for synthesizing copper nanoparticles (CuNPs) using the bacterium Pseudomonas putida. The biosynthesis process was optimized by manipulating the growth medium with various carbon sources, resulting in Pp-CuNPs approximately 91.28 nm in diameter with a surface plasmon resonance (SPR) at 550 nm. Characterization techniques as Fourier transmission infrared (FTIR) and High-resolution transmission electron microscope (HR-TEM) confirmed the successful formation and stability of the nanoparticles, with a surface charge indicating good colloidal stability. Cytocompatibility assessments demonstrated high safety of Pp-CuNPs for lung Wi38 normal cell lines, supporting their potential applications in pharmaceutical, agricultural, and environmental fields. The findings underscore the feasibility and advantages of microbial-mediated copper nanoparticles (CuNPs) production, as well as their promising biomedical and ecological applications.
正经历快速发展的一个科学领域是纳米技术。纳米技术研究的目标是开发用于制造纳米颗粒的新颖、经济、安全且有效的方法。本研究提出了一种利用恶臭假单胞菌合成铜纳米颗粒（CuNPs）的绿色、经济高效且环保的方法。通过用各种碳源操纵生长培养基对生物合成过程进行了优化，得到了直径约为91.28 nm且表面等离子体共振（SPR）在550 nm的恶臭假单胞菌铜纳米颗粒（Pp-CuNPs）。傅里叶透射红外光谱（FTIR）和高分辨率透射电子显微镜（HR-TEM）等表征技术证实了纳米颗粒的成功形成和稳定性，其表面电荷表明具有良好的胶体稳定性。细胞相容性评估表明Pp-CuNPs对肺Wi38正常细胞系具有高度安全性，支持了它们在制药、农业和环境领域的潜在应用。这些发现强调了微生物介导的铜纳米颗粒（CuNPs）生产的可行性和优势，以及它们在生物医学和生态方面的应用前景。
