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抗坏血酸的抗癌作用：并非一概而论。

Anticancer Effects of Ascorbic Acid: Not All Sides Fit All.

作者信息

Arunsi Uche O, Olugbami Jeremiah O, Oyelere Adegboyega K

机构信息

School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA.

Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332, USA.

出版信息

Cancers (Basel). 2025 Sep 1;17(17):2877. doi: 10.3390/cancers17172877.

DOI:10.3390/cancers17172877
PMID:40940976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12427553/
Abstract

Ascorbic acid (AA)is a micronutrient with concentration-dependent anticancer properties, acting either as a reactive oxygen species (ROS) scavenger or inducer. : Conventional redox-based assays such as MTS/MTT often overestimate cell proliferation due to AA's interaction with tetrazolium salts, leading to increased formazan production. To overcome this limitation, we employed the Propidium Iodide Triton X-100 (PI/TX-100) assay to evaluate AA's cytotoxic effects across a diverse panel of cancer and normal cell lines, including prostate (22Rv1, C4-2B, DU-145, LNCaP), breast (MCF-7, MDA-MB-231, MDA-MB-453), lung (A549), liver (HepG2, SK-HEP-1, Huh7), and kidney (Vero) cells. : AA significantly suppressed cancer cell viability compared to normal cells (RWPE1 and Vero), with the strongest effects observed in hormone receptor-positive lines. The relative sensitivity to AA followed distinct patterns within each cancer type. Mechanistically, AA-induced cell death involved ROS generation, lipid peroxidation, cell cycle arrest, ferroptosis, apoptosis, and downregulation of pyruvate dehydrogenase kinase 1 (PDHK1). These findings further support the potential of AA as a selective anticancer agent and highlight the importance of assay choice in evaluating its therapeutic efficacy.

摘要

抗坏血酸（AA）是一种具有浓度依赖性抗癌特性的微量营养素，可作为活性氧（ROS）清除剂或诱导剂发挥作用。传统的基于氧化还原的检测方法，如MTS/MTT，由于AA与四氮唑盐的相互作用，常常高估细胞增殖，导致甲臜生成增加。为克服这一局限性，我们采用碘化丙啶-曲拉通X-100（PI/TX-100）检测法，评估AA对多种癌症和正常细胞系的细胞毒性作用，这些细胞系包括前列腺癌（22Rv1、C4-2B、DU-145、LNCaP）、乳腺癌（MCF-7、MDA-MB-231、MDA-MB-453）、肺癌（A549）、肝癌（HepG2、SK-HEP-1、Huh7）和肾细胞（Vero）。与正常细胞（RWPE1和Vero）相比，AA显著抑制癌细胞活力，在激素受体阳性细胞系中观察到最强的作用。在每种癌症类型中，对AA的相对敏感性遵循不同模式。从机制上讲，AA诱导的细胞死亡涉及ROS生成、脂质过氧化、细胞周期阻滞、铁死亡、凋亡以及丙酮酸脱氢酶激酶1（PDHK1）的下调。这些发现进一步支持了AA作为一种选择性抗癌剂的潜力，并突出了检测方法的选择在评估其治疗效果中的重要性。

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