Komatsu Setsuko, Wai Pwint Phoo, Takeshita Tatsuya, Shiraishi Yuta
Department of Applied Science and Engineering, Fukui University of Technology, Fukui 910-8505, Japan.
Department of Applied Chemistry and Food Science, Fukui University of Technology, Fukui 910-8505, Japan.
Int J Mol Sci. 2025 Aug 27;26(17):8309. doi: 10.3390/ijms26178309.
Soybean is rich in protein and functional ingredients, which are in high demand as a food material; however, it is vulnerable to environmental stress. On the other hand, the application of chemically synthesized titanium oxide nanoparticles (TiO NPs) promoted soybean growth even under salt stress. To further enhance the growth-promoting effect of TiO NPs on soybeans, they were biologically synthesized using orange peel extract. Root elongation of soybeans suppressed by salt stress was restored to the control level by treatment with green synthesized (GS)-TiO NPs. To clarify the promoting mechanism in soybean of GS-TiO NPs under salt stress, immunoblot analysis was performed. The abundance of vacuolar H-ATPase decreased in roots by salt stress was recovered with GS-TiO NPs. In contrast, the abundance of glutathione reductase increased in roots and hypocotyls by salt stress was recovered with GS-TiO NPs. Furthermore, hydrogen peroxide production increased in roots with salt stress that was restored by treatment with GS-TiO NPs. These results suggest that GS-TiO NPs may restore soybean growth by detoxifying hydrogen peroxide, which increases under salt stress, with upregulating reactive oxygen species scavenging systems.
大豆富含蛋白质和功能性成分，作为一种食品原料需求量很大；然而，它易受环境胁迫影响。另一方面，即使在盐胁迫下，化学合成的二氧化钛纳米颗粒（TiO NPs）的应用也能促进大豆生长。为了进一步增强TiO NPs对大豆的促生长效果，采用橙皮提取物对其进行生物合成。盐胁迫抑制的大豆根伸长通过绿色合成（GS）-TiO NPs处理恢复到对照水平。为了阐明盐胁迫下GS-TiO NPs在大豆中的促进机制，进行了免疫印迹分析。盐胁迫导致根中液泡H-ATP酶丰度降低，而GS-TiO NPs可使其恢复。相反，盐胁迫导致根和下胚轴中谷胱甘肽还原酶丰度增加，GS-TiO NPs可使其恢复。此外，盐胁迫下根中过氧化氢产量增加，GS-TiO NPs处理可使其恢复。这些结果表明，GS-TiO NPs可能通过清除盐胁迫下增加的过氧化氢并上调活性氧清除系统来恢复大豆生长。
