Su Shicheng, Hu Jing, Zhu Yichun, Liang Bing, Zhu Yaping, Zhang Shuifeng, Sheng Huadong, Chen Hongbo, Xianyu Yunlei, He Chunyan
Department of Respiratory Medicine, Kunshan Hospital of Chinese Medicine, Affiliated Hospital of Yangzhou University, Suzhou, 215300, Jiangsu, China.
College of Biosystems Engineering and Food Science, Zhejiang University Hangzhou, Zhejiang, 310058, China.
Mikrochim Acta. 2025 Sep 15;192(10):668. doi: 10.1007/s00604-025-07536-9.
An all-in-one assay based on the electrical conductivity (EC) has been developed that enables the viability test and the specific detection of pathogenic bacteria, including the differentiation of (1) live/dead bacteria, (2) drug-sensitive/resistant bacteria, (3) Gram-positive/negative bacteria, and (4) specific detection of A. baumannii. Key to this assay is the modification of gold nanoparticles on the surface of bacteria that can amplify the EC signal for enhanced sensitivity and rapid detection. The amplification effect of gold nanoparticles significantly reduces the time and enhances the sensitivity for bacteria detection. This EC-based assay outcompetes traditional colorimetric and fluorescent methods owing to its rapid response, convenient operation, and low cost, which holds great promise for biosensing.
一种基于电导率（EC）的一体化检测方法已被开发出来，它能够进行活菌检测以及病原菌的特异性检测，包括区分（1）活菌/死菌、（2）药敏菌/耐药菌、（3）革兰氏阳性菌/革兰氏阴性菌，以及（4）鲍曼不动杆菌的特异性检测。该检测方法的关键在于对细菌表面的金纳米颗粒进行修饰，这可以放大EC信号以提高灵敏度并实现快速检测。金纳米颗粒的放大作用显著缩短了检测时间并提高了细菌检测的灵敏度。这种基于EC的检测方法因其响应迅速、操作简便且成本低廉，优于传统的比色法和荧光法，在生物传感方面具有巨大的应用前景。
