School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Road, Nangang District, Harbin 150001, P. R. China.
Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, Nanshan District, Shenzhen, Guangdong 518055, P. R. China.
Nano Lett. 2020 Jul 8;20(7):5036-5042. doi: 10.1021/acs.nanolett.0c01196. Epub 2020 Jun 2.
With the widespread use of antibiotics, the number of complex infection cases caused by unknown pathogens is increasing and novel antibiotics with tunable antibacterial spectra and low toxicity are highly desirable. Herein, we report that, by selecting thiol or amine, two groups with different binding affinities with gold, as anchoring groups, phenylboronic acid can be decorated on gold nanoparticles (AuNPs) with different densities, which contributes to Gram-selective antibacterial activities of the AuNPs. The AuNPs modified with amine- or thiol-tethered phenylboronic acids specifically bind to lipopolysaccharide (LPS, Gram-negative) or lipoteichoic acid (LTA, Gram-positive), respectively. By modifying AuNPs with different ratios of thiol- and amine-tethered phenylboronic acids, the resulting AuNPs show potent and tunable antibacterial activity. The AuNP-based antibacterial agents with optional Gram selectivity are promising for applications in personalized therapy.
随着抗生素的广泛应用，由未知病原体引起的复杂感染病例数量正在增加，因此人们非常希望能够获得具有可调抗菌谱和低毒性的新型抗生素。在此，我们报告称，通过选择巯基或胺基这两种与金具有不同结合亲和力的基团作为锚固基团，苯硼酸可以被不同密度地修饰到金纳米粒子（AuNPs）上，这有助于 AuNPs 具有革兰氏选择性的抗菌活性。用胺或巯基键合的苯硼酸修饰的 AuNPs 分别特异性地与脂多糖（LPS，革兰氏阴性）或脂磷壁酸（LTA，革兰氏阳性）结合。通过用不同比例的巯基和胺键合的苯硼酸修饰 AuNPs，得到的 AuNPs 表现出强大且可调的抗菌活性。基于 AuNP 的具有可选革兰氏选择性的抗菌剂有望在个性化治疗中得到应用。
