Klepukov Alexey
DiI-Lab, Moscow, Russia.
J Histochem Cytochem. 2025 Sep 14:221554251367488. doi: 10.1369/00221554251367488.
The Vibratome, or vibrating microtome allows sectioning of the mouse brain with a reliably established thickness from 50 µm, but they have a strong limitation on the size of the sample suitable for cutting. Herein is described the construction with publicly available element base (from parts for a 3D printer) a so-called 3D-vibrating microtome capable of cutting larger size brain into thin sections, and use it to investigate the limit-attainable values of the minimum slice thickness. Both of these goals have been successfully achieved. Both small mouse slices with a minimum thickness of 30 μm and large whole calf brain slices with a minimum thickness of 150 μm were obtained. Critical features of the effect of blade vibration frequency and sample feed rate on the minimum slice thickness were revealed, In the same way, a clear correlation was established between the minimum achievable thickness and its area.
振动切片机，即振动式切片机能够以50微米的可靠既定厚度对小鼠大脑进行切片，但它们在适合切割的样本大小方面有很大限制。本文描述了一种使用公开可用的元件库（来自3D打印机的零件）构建的所谓3D振动切片机，它能够将更大尺寸的大脑切成薄片，并用于研究最小切片厚度的极限可达到值。这两个目标均已成功实现。获得了最小厚度为30微米的小鼠小切片和最小厚度为150微米的小牛全脑大切片。揭示了刀片振动频率和样本进给速度对最小切片厚度影响的关键特征，同样，在可实现的最小厚度与其面积之间建立了明确的相关性。
