Pinho Jaqueline Diniz, Silva Gyl Eanes Barros, Silva Wanderley da Costa, Barbosa Eldevan da Silva, Teixeira-Júnior Antonio Augusto Lima, de Sousa Amanda Marques, Calixto José Ribamar Rodrigues, Melo Syomara Pereira da Costa, Burbano Rommel Rodriguez, Khayat André Salim, de Souza Carolina Rosal Teixeira
Professional Master's Degree in Family Health-PROFSAÚDE of the State University of Maranhão/Caxias Campus, Caxias, Maranhão, Brazil.
University State of Maranhão/Zé Doca Campus, Zé Doca, Maranhão, Brazil.
Transl Androl Urol. 2025 Aug 30;14(8):2142-2152. doi: 10.21037/tau-24-305. Epub 2025 Aug 21.
The role of small nucleolar RNAs (snoRNAs) has been investigated in the carcinogenesis of several malignancies; however, their function in penile cancer (PeCa) has not been reported. In this context, the study aimed to identify the expression profile of snoRNAs in the clinicopathological features of PeCa.
This cross-sectional observational study examined the expression profile of snoRNAs in eight patients diagnosed with PeCa and four patients with phimosis (used as controls) using the GeneChip Array, correlating it with clinicopathological characteristics. Human papillomavirus (HPV) identification was performed using nested polymerase chain reaction (PCR). analysis was conducted to characterize snoRNAs and assess their influence on the therapeutic approach for tumor types in other regions.
Two hundred and seventy snoRNAs showed differential expression between the studied groups, with the majority being overexpressed. In the Venn diagram, SNORD78 and SNORD46 had their expression identified exclusively in samples with perineural invasion and lymph node metastasis, respectively. SNORD13F was the least expressed (fold change =-10.41), while SNORD43 was the most overexpressed (fold change =9.79). analysis revealed that SNORA70 and SNORA38 were associated with a reduced therapeutic response to commonly used antineoplastic drugs in PeCa.
Despite the need to confirm the expression of these molecules in a larger number of samples and using another methodology, we suggest that these snoRNAs may serve as diagnostic, prognostic, and treatment biomarkers in PeCa.
小核仁RNA（snoRNA）在多种恶性肿瘤的致癌过程中的作用已得到研究；然而，其在阴茎癌（PeCa）中的功能尚未见报道。在此背景下，本研究旨在确定snoRNA在PeCa临床病理特征中的表达谱。
本横断面观察性研究使用基因芯片阵列检测了8例确诊为PeCa的患者和4例包茎患者（用作对照）中snoRNA的表达谱，并将其与临床病理特征相关联。使用巢式聚合酶链反应（PCR）进行人乳头瘤病毒（HPV）鉴定。进行分析以表征snoRNA并评估它们对其他区域肿瘤类型治疗方法的影响。
270种snoRNA在研究组之间表现出差异表达，大多数呈过表达。在维恩图中，SNORD78和SNORD46的表达分别仅在有神经周围侵犯和淋巴结转移的样本中被鉴定到。SNORD13F表达最少（倍数变化=-10.41），而SNORD43过表达最多（倍数变化=9.79）。分析显示，SNORA70和SNORA38与PeCa中常用抗肿瘤药物的治疗反应降低有关。
尽管需要使用另一种方法在更多样本中确认这些分子的表达，但我们认为这些snoRNA可能作为PeCa的诊断、预后和治疗生物标志物。
