Kosinska-Kaczynska Katarzyna, Zgliczynska Magdalena, Krawczyk Dominika, Piatkowska Magdalena, Balabas Aneta, Czarnowski Paweł, Goryca Krzysztof, Glinicki Piotr, Ostrowski Jerzy, Zeber-Lubecka Natalia
Department of Obstetrics, Perinatology and Neonatology, Centre of Postgraduate Medical Education, Warsaw, Poland.
Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.
Front Cell Infect Microbiol. 2025 Aug 29;15:1655581. doi: 10.3389/fcimb.2025.1655581. eCollection 2025.
Excessive body weight was associated with changes in individual microbiota. However, limited research on the impact of excessive gestational weight gain (GWG) revealed that microbiota patterns related to GWG differed from those linked to pregestational overweight or obesity.
The aim was to compare differences in the microbiota of women in the third trimester of gestation who had excessive and non-excessive weight gain during pregnancy.
Women with a singleton gestation at 34 + 0 weeks and normal pregestational body mass index were recruited to the study. Patients who were diagnosed with excessive weight gain formed the study group (n=11), while those with non-excessive weight gain formed the control group (n=10).
In cervico-vaginal samples, bacterial 16S rRNA gene sequencing demonstrated a decrease in alpha diversity, measured with the Shannon index, in the study group compared to the control group. While the difference was not statistically significant after correction for multiple testing, the Chao index showed a persistent trend toward reduced species richness in the study group. In stool samples, we identified 29 genera with differential representation between the groups, including nine overrepresented and ten underrepresented genera. The cervico-vaginal microbiota analysis detected 12 species distinguishing the study group from the controls, with four genera (, and ) being more prevalent in the study group. However, in both sites none difference was found to be statistically significant after p-value correction.
Despite small sample size, we demonstrated slight trends in microbiota composition between groups. These suggest potential differences in microbial diversity and composition associated with excessive GWG, which supports further investigation.
体重过重与个体微生物群的变化有关。然而，关于孕期体重过度增加（GWG）影响的研究有限，结果显示与GWG相关的微生物群模式与孕前超重或肥胖相关的模式不同。
比较孕期体重过度增加和未过度增加的妊娠晚期妇女微生物群的差异。
招募妊娠34 + 0周、单胎妊娠且孕前体重指数正常的妇女参与研究。被诊断为体重过度增加的患者组成研究组（n = 11），体重增加未过度的患者组成对照组（n = 10）。
在宫颈阴道样本中，细菌16S rRNA基因测序显示，与对照组相比，研究组中用香农指数衡量的α多样性降低。虽然经过多重检验校正后差异无统计学意义，但Chao指数显示研究组物种丰富度持续呈下降趋势。在粪便样本中，我们鉴定出两组间有差异的29个属，包括9个过度代表的属和10个代表性不足的属。宫颈阴道微生物群分析检测到12个可区分研究组和对照组的物种，其中4个属（、和）在研究组中更为普遍。然而，在两个部位，经p值校正后均未发现差异有统计学意义。
尽管样本量较小，但我们证明了两组间微生物群组成存在细微趋势。这些表明与过度GWG相关的微生物多样性和组成可能存在差异，这支持进一步研究。
