Brannen-Brock L R, Hall J L
Arch Androl. 1985;15(1):15-9. doi: 10.3109/01485018508986886.
Male partners of 129 infertile couples were evaluated for antisperm factors by using the hamster zona-free ovum (HZFO) test. Sperm-agglutinating activity and antifertilization factors were assessed by adding the male partner's serum to donor sperm in the HZFO-serum antibody (HA) test. When patient's HZFO scores were less than 20% of the hamster ova fertilized, fertilization by patient's serum in the HA test. Of 13 samples that failed to fertilize any of the hamster ova, nine were antibody-negative, of which seven had at least one extremely deficient sperm parameter. Of the four men with positive serum, two had normal and one had abnormal semen (one did not have a complete semen analysis). These data emphasize the need to test male partners for a decline in fertilizing capacity, which is frequently related to the presence of serum antisperm factors. These tests could be used to identify paternal and maternal components (sperm and serum) that may hinder fertilization in fertilization programs.
采用仓鼠去透明带卵母细胞（HZFO）试验，对129对不育夫妇的男性伴侣进行抗精子因素评估。在HZFO-血清抗体（HA）试验中，通过将男性伴侣的血清加入供体精子中来评估精子凝集活性和抗受精因素。当患者的HZFO评分低于受精仓鼠卵母细胞的20%时，在HA试验中用患者血清进行受精。在13个未能使任何仓鼠卵母细胞受精的样本中，9个抗体阴性，其中7个至少有一项精子参数极度缺乏。在血清阳性的4名男性中，2名精液正常，1名精液异常（1名未进行完整的精液分析）。这些数据强调了检测男性伴侣受精能力下降的必要性，这通常与血清抗精子因素的存在有关。这些检测可用于识别可能在受精程序中阻碍受精的父系和母系成分（精子和血清）。
