Dor J, Rudak E, Aitken R J
Fertil Steril. 1981 May;35(5):535-41.
The influence of antisperm antibodies on the fertilizing capacity of human sperm was investigated in a zone-free hamster egg system using heterologous and homologous antisera. The ability of these antisera to interfere with the fertilizing capacity of human spermatozoa correlated well with the antibody titers obtained using the Franklin-Dukes tube slide test. However, there was little correlation between the antifertility effects of these antisera and their activities in the gelatin and tray agglutination tests. The addition of complement to the incubation medium amplified the inhibitory activity exhibited by these antisera, although the extent of this inhibitory influence bore no relationship to the antibody titers obtained using the complement-dependent sperm-immobilization technique. The study demonstrates the importance of the hamster egg penetration test in evaluating the antifertility effect of antisperm antibodies.
利用异种和同种抗血清，在无透明带仓鼠卵系统中研究了抗精子抗体对人类精子受精能力的影响。这些抗血清干扰人类精子受精能力的能力与使用富兰克林 - 杜克斯试管玻片试验获得的抗体滴度密切相关。然而，这些抗血清的抗生育作用与其在明胶和托盘凝集试验中的活性之间几乎没有相关性。向孵育培养基中添加补体可增强这些抗血清所表现出的抑制活性，尽管这种抑制作用的程度与使用补体依赖精子固定技术获得的抗体滴度无关。该研究证明了仓鼠卵穿透试验在评估抗精子抗体抗生育作用中的重要性。
