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免疫沉淀试验中所用提取物的可变性。

Variability of extracts used in immunoprecipitin tests.

作者信息

Fink J N, Barboriak J J, Kurup V P, Scribner G H

出版信息

J Allergy Clin Immunol. 1977 Oct;60(4):238-41. doi: 10.1016/0091-6749(77)90137-3.

DOI:10.1016/0091-6749(77)90137-3
PMID:409751
Abstract

Immunodiffusion reactions of several different commercial fungal extracts from organisms were compared to those obtained from patients' environments. Sera were obtained from patients suspected of having hypersensitivity lung disease (HLD). There was a difference in reactivity among the sources of the antigen and in some lots of antigen from the same commercial supplier. The results demonstrate a wide variation in the reactivity of different antigenic extracts and emphasize the need for uniform standardization of preparations used in precipitin tests.

摘要

将几种不同的市售真菌提取物与从患者环境中获得的提取物的免疫扩散反应进行了比较。血清取自疑似患有过敏性肺病(HLD)的患者。抗原来源之间以及来自同一商业供应商的某些批次抗原之间的反应性存在差异。结果表明不同抗原提取物的反应性差异很大,并强调了沉淀素试验中所用制剂统一标准化的必要性。

相似文献

1
Variability of extracts used in immunoprecipitin tests.免疫沉淀试验中所用提取物的可变性。
J Allergy Clin Immunol. 1977 Oct;60(4):238-41. doi: 10.1016/0091-6749(77)90137-3.
2
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J Immunoassay. 1984;5(3-4):205-20. doi: 10.1080/01971528408063008.

引用本文的文献

1
Antigens of aspergillus fumigatus. 1. Purification of a cytoplasmic antigen reactive with sera of patients with aspergillus-related disease.烟曲霉抗原。1. 与曲霉相关疾病患者血清反应的一种细胞质抗原的纯化。
Clin Exp Immunol. 1981 Sep;45(3):662-71.
2
Micropolyspora faeni antigens of three commercial extracts.
Mycopathologia. 1984 Feb 15;84(2-3):165-9. doi: 10.1007/BF00436528.
3
Antigens of Aspergillus fumigatus. II. Electrophoretic and clinical studies.烟曲霉抗原。II. 电泳及临床研究。
Immunology. 1983 Aug;49(4):657-65.
4
Circulating IgG antibodies against fungal and actinomycete antigens in the sera of farmer's lung patients from different countries.来自不同国家的农民肺患者血清中针对真菌和放线菌抗原的循环IgG抗体。
Mycopathologia. 1987 May;98(2):91-9. doi: 10.1007/BF00437294.
5
Detection of IgG and IgE antibodies to Aspergillus fumigatus in human sera by immunogold assay.通过免疫金测定法检测人血清中针对烟曲霉的IgG和IgE抗体。
Mycopathologia. 1990 Jan;109(1):33-40. doi: 10.1007/BF00437004.