Zhou Tai, Liu Xue, Wu Zhimin, Lyu Riley, Peng Caizhi, Zhang Hongyi, Xia Yajing
Dermatology Department of Wuhan Third Hospital, No 241, Pengliuyang Road, Wuchang District, Wuhan, Hubei 430000, China.
Kunming Hanchen Medical Cosmetology Hospital, Kunming, Yunnan 650000, China.
ACS Omega. 2025 Aug 29;10(36):41993-42005. doi: 10.1021/acsomega.5c06769. eCollection 2025 Sep 16.
: Skin wound healing is a common clinical challenge, facing multiple obstacles including delayed healing, local hypoxia, and bacterial infection, which may lead to prolonged nonhealing or wound infection. Traditional therapeutic strategies mostly intervene in single factors, making it difficult to achieve synergistic effects. Therefore, developing an innovative approach to simultaneously address multiple issues is of great significance. : This study aims to develop a multifunctional hydrogel patch (Cu-FeO-loaded HAMA, CFH) by integrating three core functions of promoting healing, supplying oxygen, and antibacterial activity, achieving a "one-for-three" therapeutic effect to provide a more comprehensive solution for wound repair. : Cu-FeO nanoclusters (NCs) were synthesized via a hot solvent method, and CFH hydrogel patches were constructed using molds. In vitro experiments were performed to verify their biosafety, antibacterial properties, and nanozyme activity. A mouse full-thickness skin defect model was established to validate the wound-healing effect of CFH hydrogel patches in vivo. : Cu-FeO NCs were successfully synthesized, exhibiting good biocompatibility and significantly promoting the proliferation and migration of human skin fibroblasts (HSFs). Meanwhile, the material showed excellent nanozyme activity, effectively catalyzing the decomposition of hydrogen peroxide and glutathione to remarkably improve local oxygen supply in wounds. Antibacterial experiments confirmed significant inhibitory effects against and . In vivo experiments further demonstrated that CFH hydrogel patches adhered well to wounds, enabling rapid, efficient, and aseptic wound healing within 14 days. : This study successfully developed a multifunctional CFH hydrogel patch based on Cu-FeO NCs, integrating three functions of promoting cell proliferation, improving oxygen supply, and broad-spectrum antibacterial activity. It provides a safe and efficient novel therapeutic strategy for clinical wound repair, holding important clinical application value.
皮肤伤口愈合是一项常见的临床挑战，面临着包括愈合延迟、局部缺氧和细菌感染等多种障碍，这些可能导致伤口长期不愈合或感染。传统治疗策略大多针对单一因素进行干预，难以实现协同效应。因此，开发一种创新方法同时解决多个问题具有重要意义。
本研究旨在通过整合促进愈合、供氧和抗菌活性这三种核心功能，开发一种多功能水凝胶贴片（负载铜铁氧化物的透明质酸甲基丙烯酸酯，CFH），实现 “一药三用” 的治疗效果，为伤口修复提供更全面的解决方案。
通过热溶剂法合成了铜铁氧化物纳米簇（NCs），并使用模具构建了CFH水凝胶贴片。进行体外实验以验证其生物安全性、抗菌性能和纳米酶活性。建立小鼠全层皮肤缺损模型以验证CFH水凝胶贴片在体内的伤口愈合效果。
成功合成了铜铁氧化物纳米簇，其表现出良好的生物相容性，并显著促进人皮肤成纤维细胞（HSF）的增殖和迁移。同时，该材料显示出优异的纳米酶活性，有效催化过氧化氢和谷胱甘肽的分解，显著改善伤口局部供氧。抗菌实验证实对[具体细菌1]和[具体细菌2]有显著抑制作用。体内实验进一步表明，CFH水凝胶贴片与伤口贴合良好，能够在14天内实现快速、高效且无菌的伤口愈合。
本研究成功开发了一种基于铜铁氧化物纳米簇的多功能CFH水凝胶贴片，整合了促进细胞增殖、改善供氧和广谱抗菌活性这三种功能。它为临床伤口修复提供了一种安全有效的新型治疗策略，具有重要的临床应用价值。
